Cloning and sequence analysis of Actin gene fragment from Talinum paniculatum Gaertn
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 6
Abstract
"In order to provide reference gene for studying the expression pattern of functional genes in Talinum paniculatum，the cDNA fragment of Actin from Talinum paniculatum；was amplified by RT-PCR，by adopting degenerated primers designed according to the conserved region of the Actin genes which were submitted to NCBI database. The cDNA fragment was inserted into the pGEM-T vector and sequenced. The cDNA sequence was analyzed by online bioinformatics software. Our result showed that this actin cDNA includes 598 base pairs，encoding a partial actin with 198 amino acids. Sequence alignments showed that it shares over 85% nucleotide sequence identity and 86% amino acid sequence identity with other plant actins. This study indicated that a new actin cDNA fragment was isolated from Talinum paniculatum. This cDNA was named TpActin1 and submitted to GenBank （accession number: MH33309）."
Authors and Affiliations
Yu-yan YE, Li-xiang YANG
Keywords
Related Articles
- EP ID EP600573
- DOI 10.16768/j.issn.1004-874X.2018.06.004
- Views 39
- Downloads 0
Impact of pesticide use intensity on the occurrence area of crop diseases and insect pests —Empirical analysis based on the random effects model
"A random effects model was used to analyze the influence of pesticide use intensity on the occurrence area of crop diseases and insect pests using the unit root tests with the panel data set covering the 30 provinces，...
Identification and cluster analysis of stripe rust resistance in 22 wheat varieties in Sichuan province
The wheat varieties cultivated in 22 Sichuan areas approved by Sichuan Province from 2010 to 2017 were selected,and their resistance to stripe rust was identified and high-resistance varieties were screened. Then, the fi...
Study on diversity and color temporal and spatial distribution of early spring wildflower plant in Handan city
In this article the early spring wild flowering plants in the urban area of Handan were the subjects.The using diversity and flower color and its spatio-temporal distribution of wild flowering plants in early spring were...
Flower bud differentiation and development characteristics of Camellia gauchowensis Chang
To understand the differentiation of flower bud of Camellia gauchowensis Chang, and to grasp the characteristics of its differentiation, and provide technical support for the regulation and control of the flowering perio...
High yield cultivation mathematical model and its application on maize new hybrid Xixiangban99
In this experiment，quadratic orthogonal regressive rotation design of five factors were used to study the effects of planting factors such as sowing date，density，nitrogen，phosphorus and potassium on the yield stabili...