Cloning and sequence analysis of Actin gene fragment from Talinum paniculatum Gaertn
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 6
Abstract
"In order to provide reference gene for studying the expression pattern of functional genes in Talinum paniculatum，the cDNA fragment of Actin from Talinum paniculatum；was amplified by RT-PCR，by adopting degenerated primers designed according to the conserved region of the Actin genes which were submitted to NCBI database. The cDNA fragment was inserted into the pGEM-T vector and sequenced. The cDNA sequence was analyzed by online bioinformatics software. Our result showed that this actin cDNA includes 598 base pairs，encoding a partial actin with 198 amino acids. Sequence alignments showed that it shares over 85% nucleotide sequence identity and 86% amino acid sequence identity with other plant actins. This study indicated that a new actin cDNA fragment was isolated from Talinum paniculatum. This cDNA was named TpActin1 and submitted to GenBank （accession number: MH33309）."
Authors and Affiliations
Yu-yan YE, Li-xiang YANG
Keywords
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- EP ID EP600573
- DOI 10.16768/j.issn.1004-874X.2018.06.004
- Views 42
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