Co-Administration of Anti-Angiogenic Peptide and DNA Vaccine in Cervical Cancer Tumor Model
Journal Title: International Journal of Cancer Management - Year 2017, Vol 10, Issue 3
Abstract
Background Cervical cancer is one of the main causes of women’s death in the world. Human papilloma virus (HPV) types 16 and 18 have been known as a cause of more than 2/3 of cervical cancers. New combination treatment strategies based on immune responses against viral antigens are likely to be beneficial. Considering the important role of angiogenesis in nutrition and oxygenation of tumor cells, the inhibition of this process can help tumor clearance. Objectives In this study, co-administration of a plasmid encoding immune stimulatory epitopes of E6-E7-L1 genes of HPV with an anti-angiogenic peptide derived from Endostatin in tumor mice model was examined. Methods C57BL/6 mice were injected subcutaneously with TC-1 tumor cells and monitored for tumor progression. At exact time points, tumor size was determined. Then they were injected by pIRES-E6/E7/L1 as DNA vaccine, and anti angiogenic peptide. Relative tumor volume measurements and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was carried out in order to investigate therapeutic antitumor effects of vaccine and peptide. Results Based on the results, the group of mice that received vaccine and vaccine-peptide had significant inhibition rate of tumor growth in comparison with control groups (P < 0.05). Lymphocyte proliferation responses against the vaccine in the related groups were significantly higher than negative control group (P < 0.05). Conclusions In general, it could be concluded that the co-administration of DNA vaccine due to the good immunogenicity and suitable inhibitory effect of peptide in reduction of tumor size, shows higher efficacy and can be considered as a new therapeutic strategy.
Authors and Affiliations
Parisa Saadat, Hoorieh Soleimanjahi, Seyed Mohsen Asghari, Maryam Fazeli, Hadi Razavinikoo, Hesam Karimi
Keywords
Related Articles
- EP ID EP237334
- DOI 10.5812/ijcm.4723
- Views 67
- Downloads 0
Co-Administration of Anti-Angiogenic Peptide and DNA Vaccine in Cervical Cancer Tumor Model
Background Cervical cancer is one of the main causes of women’s death in the world. Human papilloma virus (HPV) types 16 and 18 have been known as a cause of more than 2/3 of cerv...
Application of Cold Atmospheric Plasma (CAP) in Cancer Therapy: A Review
Background Recently the use of cold atmospheric plasma (CAP) for the treatment of cancers called “Plasma Oncology” has gained promising results. The developments have raised the h...
Extracranial Extrarenal Rhabdoid Tumor: A Case Report
Introduction Rhabdoid Tumor is one of the rare and aggressive malignant tumors of childhood that was introduced as a subgroup of the rhabdomyosarcomatoid of the Wilm’s tumor in 19...
Immunohistochemical Study of HER2/neu Expression in Colorectal Cancer and its Relation to other Clinicopathological Criteria and Prognostic Factors
Background: Human epidermal growth factor receptor 2 (HER2) overexpression and amplification have been studied as a therapeutic and prognostic target in a number of tumors although conflicting data exist about the incide...
Intact Expression of Hypoxia-Inducible Factor 1α (HIF-1α) Gene in HeLa Cell Line Following Treatment with Lactobacilli Supernatants
No Abstract