Cold-enhanced gene expression of the foliar thiol-specific antioxidant protein in triticale (x Triticosecale Wittmack) seedlings resistant to the pink snow mould resulted from Microdochium nivale (Samuels and Hallett) infection
Journal Title: Acta Biologica - Year 2015, Vol 22, Issue 846
Abstract
Pink snow mould resulting from Microdochium nivale (Samuels & I.C. Hallett) infection is one of the most wide spread and serious diseases of winter cereals and amongst them triticale (x Triticosecale, Wittm.), the man-made crop with great yielding potential even in poor environmental conditions. Therefore, we aimed to identify snow mould-resistant genotypes and gene/s potentially involved in the increased resistance. In our experimental design we have used two parental cultivars and four descendent doubled haploid (DH) lines, with the highest and the lowest cold-induced snow mould resistance in our multi-season cold chamber tests. In seedlings leaves of those genotypes we verified the effect of cold-hardening (4 weeks at 4°C) on the relative expression of selected genes: catalase 1 (Cat1), pathogen-esis-related protein 1 (PR1) and thiol-specific antioxidant protein (Tsa), using quantitative reverse transcriptase Real-Time PCR (qPCR). In present analysis, the genotype and the interaction of genotype–hardening had significant effects on the mean relative expression of all genes studied. The relative Cat1 and Tsa genes expression measured in non-hardened plants showed weak positive correlation with triticale seedlings pink snow mould susceptibility. Contrary, in cold-hardened plants we found negative correlation of the relative PR1 and Tsa genes expression with the susceptibility. Based on our results, we suggest that cold-enhanced Tsa gene expression in leaves of winter triticale seedlings could be possibly involved in plant preparing against following M. nivale infection but it should be verified during further inoculation experiments. According to our knowledge, the expression of Tsa gene in triticale was for the first time confirmed by us.
Authors and Affiliations
Gabriela Gołębiowska-Pikania, Elżbieta Golemiec
Keywords
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- EP ID EP171320
- DOI DOI 10.18276/ab.2015.22-08
- Views 31
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