Collagen type I and III study in the gastroesophageal junction in the patients with hiatal hernia
Journal Title: Хірургія України - Year 2019, Vol 0, Issue 1
Abstract
The aim — to study the collagen I and III composition in samples of the phrenoesophageal ligament in patients with and without hiatal hernia. Materials and methods. The composition of collagen type I and type III was studied in samples of the phrenoesophageal ligament in 18 patients with gastroesophageal reflux disease who underwent hiatal hernia repair. Intraoperative biopsy was performed on all patients (main group). For comparison, samples of the phrenoesophageal ligament were collected from 14 cadavers (control group). In the main group there were 8 (44.4 %) men and 10 (55.6 %) women, in the comparison group — 6 (42.9 %) and 8 (57.1 %), respectively (p > 0.05). The average age in the groups was 49.0 ± 15.4 and 49.3 ± 9.6 years, respectively (p > 0.05). The body mass index did not significantly differ in the groups (p > 0.05). Patients with diseases associated with collagen synthesis, smokers and individuals who had previously undergone an operation in the area of the esophagogastric ligament were excluded from the study. The amount of type I and III collagen was determined with primary monoclonal antibodies to the corresponding proteins. To quantify the results of immunohistochemical reactions in sections with a 400fold increase in the microscope, the number of cells in which immunoperoxidase marks (positive staining) are detected in 10 fields of view selected at random (> 200 cells) is detected. The calculation of the results was carried out according to the HSCORE formula. Results and discussion. The content of collagen type I and type III in the ligaments in patients with hiatal hernia was 49.1 % and 29.3 %, respectively, lower than in the control group (p < 0.01). Conclusions. Patients with large and giant hernias has a connective tissue synthesis defect. The quality of phrenoesophageal ligament can be an etiological factor for the hiatal hernia occurrence. This factor should be considered when selecting a method of laparoscopic cruroraphy.
Authors and Affiliations
V. V. Grubnyk, T. A. Savenko
Keywords
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- EP ID EP564389
- DOI 10.30978/SU2019-1-34
- Views 106
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