Abstract

The collagenase administration through umbilical artery is the effective treatment for Retained Fetal Membrane (RFM) in dairy cows. RFM was treated with collagenase enzyme through jugular vein as it is easy route than previous study of experimenting on umbilical arteries which is very difficult in a delayed case in field conditions in bovines. The study was conducted in placental tissues of bovines that are presented within 12 to 24 hours after parturition to Obstetrics Unit of Madras Veterinary College, Chennai to determine the efficacy of enzyme and dose effectiveness prior to actual treatment with field cases for therapeutic purposes. The experimental animals were divided in to four different treatment groups (Gp I:n=7; Gp II:n=15; Gp III:n=15 and Gp IV:n=15) and placental tissue samples were collected accordingly from all groups.The dose of 1800 CDU collagenase had significantly higher (P<0.01) concentrations of placental tissue hydroxyproline (35.00 ± 0.63 to 36.31 ± 0.31 μg/ml) and placental tissue total proteinrelease (1.68 ± 0.02 to 1.73 ± 0.02 g/dl), than the remaining groups. The data were collected, compared and analyzed; further, the findings showed that 1800 CDU dose was selected for the RFM treatment

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