COMBINATIONAL EFFECTS OF ETHYLACETATE EXTRACT OF PICRIA FEL-TERRAE LOUR AND DOXORUBICIN ON T47D BREAST CANCER CELLS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 7
Abstract
Objective: To evaluate the effects of ethyl acetate extract (EAE) of Picria fel-terrae Lour. Leaves and combination with doxorubicin on cytotoxicity, cell cycle, apoptotis and suppression of cyclin D1 and Bcl-2 expression on T47D cell lines.Methods: The in vitro cytotoxicity effects were determined using MTT assay. Analysis of cell cycle distribution was performed using flow cytometer and the data was analyzed using ModFit LT 3.0 program. Apoptotis assay was analyzed using flow cytometer using Annexin V. The suppression of cyclin D1 and Bcl-2 expression on T47D cell lines was identified using immune cytochemistry.Results: Cytotoxicity activity and combination of EAE with doxorubicin were evaluated using the MTT assay. The combination represents higher inhibitory effect on cell growth than the single treatment of doxorubicin on T47D cell lines. The combination changes the accumulation of cell cycle phase from G0-G1. The combination also increases apoptosis. The combination also showed suppression of cyclin D1 and Bcl-2 expression in T47D cell lines.Conclusion: Based on the results, EAE is potential to be developed as co-chemotherapeutic for breast cancer by inducing apoptosis, cell cycle arrest and suppressing cyclin D1 and Bcl-2 expression. However, the molecular mechanism needs to be explored further. Â
Authors and Affiliations
Denny Satria, Mainal Furqan, Sumadio Hadisahputra, Rosidah .
Keywords
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