Comparación de los métodos de preservación de especímenes Anopheles (Diptera: Culicidae) para la extracción de ADN
Journal Title: Revista Científica Salud Uninorte - Year 2011, Vol 27, Issue 1
Abstract
Abstract Introduction: Various methodologies have been reported for long term specimen preser- vation, which protect the DNA from degradation allowing its posterior analysis in molecu- lar studies. However, the effectiveness of the preservation methods may vary among insect groups; therefore, it is convenient to determine the preservation method to be used with a particular group before performing molecular studies with a large number of specimens. Objetives: An experimental comparative study was conducted to evaluate different pres- ervation treatments for Anopheles albimanus, to know their utility in conserving the quantity and quality of DNA to be used in subsequent molecular studies. Methods: DNA was extracted from each specimen, it was then quantified by spectropho- tometer and the Internal Transcribed Spacer 2 (ITS2) was amplified by PCR. Results: ITS2 amplification was obtained in all specimens without previous preservation, in 60% of those preserved in sílica gel and in 20% of those preserved at -80°C and in 70% ethanol. A significant difference (p<0.05) was found between amplification proportions. Amplification was not obtained in specimens preserved frozen at -20°C and not correla- tion by logistic regression was observed between the DO260/DO280 index and DNA con- centration of specimens presenting ITS2 amplification. Conclusions: The data suggest that keeping specimens in silica gel or frozen at -80°C may be the best conditions to preserve Anopheles specimens for subsequent molecular studies. Key words: Anopheles, malaria, DNA, samples preservation, polymerase chain reaction.
Authors and Affiliations
Tatiana Castaño Seúlveda, Paola sánchez Zapata, Lorena Viana Guerra, Margarita Correa Ochoa, Lina Gutierrez Builes, Maria Zapata Tamayo
Keywords
Related Articles
- EP ID EP86574
- DOI -
- Views 192
- Downloads 0
Comparación de los métodos de preservación de especímenes Anopheles (Diptera: Culicidae) para la extracción de ADN
Abstract Introduction: Various methodologies have been reported for long term specimen preser- vation, which protect the DNA from degradation allowing its posterior analysis in molecu- lar studies. However, the ef...
Importance of the motor development in terms of the evolutionary process of language and cognition in children between ages 3 to 7 in the city of Barranquilla (Colombia)
Introduction: Knowing the development sequentiality in the first years of children life establish appropriate intervention strategies. Objetive: The purpose of this paper is to determine the relationship between motor d...
Quality of life in people with HIV/AIDS belonging to non- governmental organization. Medellín (Colombia), 2009
Abstract Objective: To describe the quality of life in people with HIV/AIDS in Medellín, according the MOSSF-36 and the stories of individuals living with HIV/AIDS. Materials and methods: Mixed in all subjects wi...
Una reflexión acerca del Modelo de Investigación Biomédica
Resumen El modelo biomédico es una construcción epistemológica histórica que se ha gestado como consecuencia de la relación conceptual establecida entre el concepto salud-enfermedad y la investigación científica,...
Relación entre conductancias de sodio y potasio al estilo Hodgkin y Huxley y su efecto en la propagación de potenciales de acción a 40°C
Resumen Objetivo: Determinar, a partir de simulación computacional, la respuesta de un axón no mielinado a un estímulo de corriente, para diferentes valores de GNa y GK tales que 0S/ cm2<GNa≤6.4S/cm2 y 0S/cm2<GK≤2...