COMPARATIVE ANALYSIS OF ANTIMICROBIAL ACTIVITY OF LEAF EXTRACTS FROM THREE SPECIES OF GENUS HYDROCOTYLE ON PATHOGENS
Journal Title: European Journal of Pharmaceutical and Medical Research - Year 2017, Vol 4, Issue 10
Abstract
Medicinal plants have been a major source of therapeutic agents for alleviation and cure diseases. The antimicrobial activity of the extracts of three species of genus Hydrocotyle was analyzed by using well diffusion method paper disk diffusion method and minimum inhibitory concentration. In ethanoic extract of H. ramiflora, maximum inhibition zone diameter among all the tested microorganisms was obtained in Enterobactercloaceaewith diameter of 6.1±0.5 mm. H. ramiflorashowed maximum inhibition zone with diameter of 6.3±0.3 mm in Escherichiacoli, 6.1±0.3 mm in Enterobacteraerogenes,and 6.4±0.4 mm in Klebsiellapneumoniae. For all the tested microorganisms, ethanol extracts of H. maritimashowed maximum antibacterial activity in E. coli.Maximum inhibition zone diameter of H. maritimawere obtained in E. cloaceaeand in E. coliwith diameter 6.2±0.4 mm and 6.4±0.5 mm, respectively. In ethanolic extract of H. nepalensis, maximum inhibition zone diameter among all the tested microorganisms was obtained in E. coliDD12 with diameter of 6.4±0.5 mm (Table 3). H. nepalensisshowed maximum inhibition zone with diameter of 6.2±0.4 mm in E. cloaceae, 6.3±0.4 mm in E.aerogenes,and 6.3±0.3 mm in K. pneumoniae. Although the two stock strains are different, there was not significant differences (p> 0.05). In addition, for each strain,there was not significant differences among four various concentrations (p> 0.05).
Authors and Affiliations
Prof. Man Kyu Huh
Keywords
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