Comparative Analysis of Genetic Diversity among 24 Groundnut Genotypes using Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) Marker
Journal Title: International Journal of Agricultural Science and Research (IJASR) - Year 2017, Vol 7, Issue 3
Abstract
Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) were assayed to determine the genetic diversity of 24 genotypes of groundnut grown in Udaipur region of Rajasthan. RAPD and ISSR analysis showed high percent of polymorphism 90.23 and 85.71 respectively. The average PIC and Jaccard’s similarity coefficient values for RAPD and ISSR was 0.216 ranging from 0.0319 to 0.449; 0.07 to 0.37 with an average of 0.300 and 0.63-0.91 with an average of 0.79; 0.54-0.90 with an average of 0.72 respectively. Based on the dendrogram generated by UPGMA method and PCA, most of the genotypes could be divided into four main clusters for RAPD and five clusters for ISSR.
Authors and Affiliations
Dhwani Sharma, Arunabh Joshi, Devendra Jain
Keywords
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