Comparative Analysis of Molecular Structure, Function and Expression of Buffalo (Bubalus bubalis) Toll-Like Receptor 9
Journal Title: Journal of Buffalo Science - Year 2013, Vol 2, Issue 2
Abstract
Toll-like receptor 9 (TLR9) has been characterized as a receptor that recognizes unmethylated CpG motif and triggers a pro-inflammatory cytokine response that influences both innate and adaptive immunity. Buffalo is an economically important livestock species in many Asian and Mediterranean countries, but there is little information available on its TLR9 structure and response to stimulation with its agonist CpG-ODNs. Hence in this study, we report the analysis of newly sequenced buffalo TLR9 gene fragment. In this study, buffalo TLR9 amino acid sequence revealed close association of TLR9 proteins within other bovines and small ruminants; but high divergence from other species. Multiple alignment of deduced amino acid sequence of Bubalus bubalis TLR9 with other species showed that 156/201 (74.28%) amino acids were conserved in all species. Leucine rich repeat (LRR) motifs in the ectodomain of TLR9 are responsible for molecular recognition of its agonist. The LRR pattern of Bubalus bubalis TLR9 protein was predicted towards N-terminal sequence and was found to be conserved among all species except Rattus norvegicus and Equus caballus. Blast analysis of buffalo TLR9 sequence with single nucleotide polymorphisms (SNPs) database revealed 13 SNPs out of which 7 were cds-synonymous and 6 were of the functional significance. Furthermore, kinetics of TLR9 and proinflammatory IL-1 and TNF- cytokine expression by buffalo PBMCs influenced by CpG-ODN is also discussed.
Authors and Affiliations
Anju Manuja, Balvinder K. Manuja, Ranjit S. Kataria, Ramesh K. Sethi and Raj K. Singh
Keywords
Related Articles
- EP ID EP33561
- DOI -
- Views 355
- Downloads 0
Prepubertal Buffalo (Bubalus bubalis) Leydig Cells: Isolation, Culture and Characterization
Water buffalo (Bubalus bubalis) is an economically important livestock species in India. Male buffaloes display delayed sexual maturity as compared to the bulls (Bos taurus). Serum testosterone level, the key regulator...
Biochemical Profile of Milk of Buffalo (Bubalus bubalis), Cow (Bos taurus) and Goat (Capra hircus): Potential Candidates for Supporting the Growth of Leishmania donovani Promastigotes in Culture Medium as Alternative to Fetal Bovine Serum (FBS)
The milk of cow (Bos taurus), buffalo (Bubalus bubalis), and goat (Capra hircus) were investigated for the biochemical profile such as glucose, total protein, triglycerides, cholesterol and calcium. Although used as pot...
Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques
This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm cells were incubated in Tyrode albumin lactate pyruvate medium (TALP) in absence of ca...
Usage of Saliva as Alternative Biological Fluid to Serum for Minerals, Energetic and Hormones Assessment in Lactating Egyptian Water Buffaloes
Blood sample is the most common biological fluid utilized for diagnosis and monitoring of diseases. Saliva contains locally produced substances as well as serum component, so the aim of this study is to compare the prof...
Ultrasound-Guided Paravertebral Regional Anesthesia in Water Buffalo
Adequate flank regional anaesthesia was achieved using ultrasound-guided nerve block of the 13th thoracic, 1st and 2nd lumbar spinal nerves after their exits from their intervertebral foraminae with only 5 ml of lidocai...