Comparative Analysis of Molecular Structure, Function and Expression of Buffalo (Bubalus bubalis) Toll-Like Receptor 9
Journal Title: Journal of Buffalo Science - Year 2013, Vol 2, Issue 2
Abstract
Toll-like receptor 9 (TLR9) has been characterized as a receptor that recognizes unmethylated CpG motif and triggers a pro-inflammatory cytokine response that influences both innate and adaptive immunity. Buffalo is an economically important livestock species in many Asian and Mediterranean countries, but there is little information available on its TLR9 structure and response to stimulation with its agonist CpG-ODNs. Hence in this study, we report the analysis of newly sequenced buffalo TLR9 gene fragment. In this study, buffalo TLR9 amino acid sequence revealed close association of TLR9 proteins within other bovines and small ruminants; but high divergence from other species. Multiple alignment of deduced amino acid sequence of Bubalus bubalis TLR9 with other species showed that 156/201 (74.28%) amino acids were conserved in all species. Leucine rich repeat (LRR) motifs in the ectodomain of TLR9 are responsible for molecular recognition of its agonist. The LRR pattern of Bubalus bubalis TLR9 protein was predicted towards N-terminal sequence and was found to be conserved among all species except Rattus norvegicus and Equus caballus. Blast analysis of buffalo TLR9 sequence with single nucleotide polymorphisms (SNPs) database revealed 13 SNPs out of which 7 were cds-synonymous and 6 were of the functional significance. Furthermore, kinetics of TLR9 and proinflammatory IL-1 and TNF- cytokine expression by buffalo PBMCs influenced by CpG-ODN is also discussed.
Authors and Affiliations
Anju Manuja, Balvinder K. Manuja, Ranjit S. Kataria, Ramesh K. Sethi and Raj K. Singh
Keywords
Related Articles
- EP ID EP33561
- DOI -
- Views 343
- Downloads 0
Study on Availability of Various Macro and Micro-Minerals in Lactating Buffaloes under Field Conditions of Sabarkantha District of Gujarat
A study was conducted in the Sabarkantha district of Gujarat to assess the status of some macro and microminerals in lactating buffaloes. Feeds and fodder samples were collected from 17 representative villages of the dis...
Assessing Minerals Status of Dairy Animals in South-West Zone of Punjab
A study was carried out to assess dietary macro and micro-minerals status of milch buffaloes in Firozpur, Mansa and Muktsar districts, under South-West Zone of Punjab. Feeds and fodder samples were collected at random...
Current View of the Buffalo Industry
It is common in buffalo industry that exist a separation between scientists, breeders and government; as a consequence it is very easy to make mistakes for lack of knowledge, for the appreciation of that the buffalo...
Metagenomic Analysis of Uterine Microbiota in Postpartum Normal and Endometritic Water Buffaloes (Bubalus bubalis)
In Indian subcontinent the water buffalo (Bubalus bubalis) is one of the important livestock animals. As in cows, postpartum infection like endometritis in dairy buffaloes is major cause for the economic loss in the dai...
Isolation and Characterization of Mannheimia varigena from a Murrah Buffalo
The present study was aimed to isolate and characterize phenotypically as well as genotypically bacterial strain from tracheal froth of a Murrah buffalo on the Government livestock farm (GLF) located at Hisar, Haryana....