Comparative Analysis of Monosaccharide and Disaccharide Using Different Instrument Refactometer and Polarimeter
Journal Title: International Journal of Research in Chemistry and Environment (IJRCE) - Year 2012, Vol 2, Issue 4
Abstract
Comparative analysis of monosaccharide and disaccharides were carried out with the aid of refractometer and polarimeter, statistical analysis were made using linear regression equation and analysis of variance (ANOVA). Refractometry was more sensitive (R2 =0.99) than polarimetry whose sensitivity is (R2 =0.94). The observed significant difference among the methods is (P ≤ 0.05). The results show that retractometry with R2 =0.99 is more sensitive than polarimeter.The sensitivity of R2 =09.99 is similar for all the sugars and their standard mixtures suggesting that without prior separation refractometry can be used for the determination of any of these sugars and their mixtures Polarimeter was less sensitive in determining the fructose level.
Authors and Affiliations
Anyika C. , Okonkwo I. , Ejike N.
Keywords
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