Comparative analytic study of exotoxin A gene production among ESBL and non- ESBL producing clinical isolates of Pseudomonas aeruginosa
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 17, Issue 2
Abstract
Exotoxin A is an extracellular enzyme that is produced by most clinical strains of Pseudomonas aeruginosa which inactivates and inhibits the protein biosynthesis. The present study aims to detect any possible correlation or any significant association between the production of exotoxin A and antibiotic resistance among P. aeruginosa clinical isolates or they can act independently, which might have implications for treatment and control. A Prospective study done on 60 isolates of Pseudomonas aeruginosa which included 30 ESBL and 30 Non- ESBL producers were randomly selected from total 203 isolates from various clinical samples in a tertiary care hospital , Puducherry. All isolates were screened for ESBL production using Phenotypic confirmatory disc diffusion test. Detection of Exotoxin A (toxA) gene expression were done by conventional PCR..Out of 60 clinical isolates of Pseudomonas aeruginosa showed 70% (42/60) positivity for exotoxin A by PCR. Among 30 ESBL positive isolates, 76.7% (23/30) were positive and among 30 ESBL negative isolates 63.3% ( 19/30) were positive for toxA gene production.In our study, 70% positivity of toxA gene in clinical isolates of Pseudomonas aeruginosa were obtained of which 76.7% were from ESBL and 63.3% were from non-ESBL producers. Henceforth, the association between exotoxin A gene expression and resistance mechanism like ESBL production was not statistically significant in our study, which may indicate that there need not be always a direct correlation between virulence gene and antibiotic resistance and they may act independently.
Authors and Affiliations
Pramodhini S, Anitha TS, Umadevi S1
Keywords
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- EP ID EP363776
- DOI 10.9790/0853-1702124146
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