COMPARATIVE DIAGNOSIS OF TYPHOID FEVER BY POLYMERASE CHAIN REACTION AND WIDAL TEST IN SOUTHERN DISTRICTS (BANNU, LAKKI MARWAT AND D.I.KHAN) OF KHYBER PAKHTUNKHWA, PAKISTAN
Journal Title: Acta Scientifica Malaysia - Year 2017, Vol 1, Issue 2
Abstract
Typhoid fever is a major dilemma in developing nations, despite the antibiotic use and new antibiotics discovery. Blood culture and serological tests (especially Widal test) that are regularly performed in Pakistan for diagnosis provide objectionable levels of false negative and false positive results, respectively. The present study was carried out at Kohat University of science and technology, Kohat, Pakistan from January 2013 to December 2013. A polymerase chain reaction (PCR) assay was compared with Widal test and blood culture for blood samples of 96 patients. In this study, blood from 25 healthy individuals was collected as a negative control. The detection rate of PCR was maximum (64.5%) followed by Widal test (26%) and blood culture (14.5%). Among the 82 (85.4%) samples that were found negative with blood culture, 48 (58.5%) were successfully detected by PCR and 11 (13.4%) were positive for the Widal test. Findings of this study showed that PCR is a fast and reliable technique for diagnosis of typhoid fever in suspected cases, as compared to frequently used conventional techniques like blood culture and Widal test.
Authors and Affiliations
Irfan Ullah Khan, Sanaullah Sajid, Asad Javed, Sedra Sajid, Shafi Ullah Shah, Shahid Niaz Khan, Kalim Ullah
Keywords
Related Articles
- EP ID EP261859
- DOI 10.26480/asm.02.2017.12.15
- Views 81
- Downloads 0
ASSOCIATION OF BLOOD LEAD LEVELS AND WORKING MEMORY ABILITY OF PRIMARY SCHOOL CHILDREN SURROUNDING EX-COPPER MINING AREA IN RANAU, SABAH (MALAYSIA)
A cross sectional study on the association of Blood Lead Levels (BLL) and Working Memory (WMICS) ability of primary school children surrounding ex-copper mining area in Ranau, Sabah was conducted from September 2012 till...
EVALUATION OF GREEN SOLUTION POTENTIALS OF UNRIPE BANANA (MUSA SAPIENTUM) PEEL BIOMASS
In the present study, the green solution potentials of unripe banana (musa sapientum) peel biomass were evaluated by using the adsorbent for the removal of Cd (II) and Cr (VI) from aqueous solution. The batch method was...
EVALUATION OF COSMETICS FOR THEIR POTENTIAL CONTAMINANTS AND DRUG RESISTANT MICROORGANISMS
Cosmetic products are those formulations which are applied on different parts of body including teeth, nails, face and hair. These are used to clean body and improve appearance. On the basis of area on which cosmetics ar...
Environmental effect of Sudan I-IV: adsorption behaviors and potential risk on soil
Sudan dyes (Table 1) [1], a class of synthetic azo dyes and classified as category 3 carcinogens by the International Agency for Research on Cancer[2], have been received considerable attention all over the world, especi...
BLOOD LEAD CONCENTRATION AND WORKING MEMORY ABILITY ON MALAY PRIMARY SCHOOL CHILDREN IN URBAN AND RURAL AREA, MALACCA
The most lead concentrations in the environment come from human activities such as burning fossil fuels, mining and manufacturing. Once lead enters into environment, it does not break down, but lead compounds were change...