COMPARATIVE DIAGNOSIS OF TYPHOID FEVER BY POLYMERASE CHAIN REACTION AND WIDAL TEST IN SOUTHERN DISTRICTS (BANNU, LAKKI MARWAT AND D.I.KHAN) OF KHYBER PAKHTUNKHWA, PAKISTAN
Journal Title: Acta Scientifica Malaysia - Year 2017, Vol 1, Issue 2
Abstract
Typhoid fever is a major dilemma in developing nations, despite the antibiotic use and new antibiotics discovery. Blood culture and serological tests (especially Widal test) that are regularly performed in Pakistan for diagnosis provide objectionable levels of false negative and false positive results, respectively. The present study was carried out at Kohat University of science and technology, Kohat, Pakistan from January 2013 to December 2013. A polymerase chain reaction (PCR) assay was compared with Widal test and blood culture for blood samples of 96 patients. In this study, blood from 25 healthy individuals was collected as a negative control. The detection rate of PCR was maximum (64.5%) followed by Widal test (26%) and blood culture (14.5%). Among the 82 (85.4%) samples that were found negative with blood culture, 48 (58.5%) were successfully detected by PCR and 11 (13.4%) were positive for the Widal test. Findings of this study showed that PCR is a fast and reliable technique for diagnosis of typhoid fever in suspected cases, as compared to frequently used conventional techniques like blood culture and Widal test.
Authors and Affiliations
Irfan Ullah Khan, Sanaullah Sajid, Asad Javed, Sedra Sajid, Shafi Ullah Shah, Shahid Niaz Khan, Kalim Ullah
Keywords
Related Articles
- EP ID EP261859
- DOI 10.26480/asm.02.2017.12.15
- Views 82
- Downloads 0
DETERMINATION OF POLYCHLORINATED BIPHENILS IN THE SOIL, ATMOSPHERIC DEPOSITION AND BIOINDICATOR SAMPLES IN THE MERIC-ERGENE RIVER BASIN, TURKEY
The Meric-Ergene Basin, located on the north-western part of Turkey, begins with the Istanbul provincial border in the east and covers the border area with Bulgaria and Greece borders in the west. The industry developed...
A STUDY ON SOME FUNDAMENTAL CHARACTERISTICS OF SEQUENCES OF SOFT REAL NUMBERS
The main aim of this article is to discuss soft limit, convergent sequences, Soft boundedness, and Soft convergent. Divergent criterion. Soft monotone sequences, Soft squeezness, Soft Cauchy Sequence, Soft Cauchy bounded...
ANTIBACTERIAL ACTIVITIES OF Piper sarmentosum (KADUK) METHANOLIC EXTRACT
Piper sarmentosum or locally known as Kaduk is a tropical herbal plant that is well established for many traditional medicinal purposes. The antibacterial properties of methanolic extract for P. sarmentosum leaves have b...
Environmental effect of Sudan I-IV: adsorption behaviors and potential risk on soil
Sudan dyes (Table 1) [1], a class of synthetic azo dyes and classified as category 3 carcinogens by the International Agency for Research on Cancer[2], have been received considerable attention all over the world, especi...
EFFECT OF ROW SPACING ON YIELD OF SHORT DURATION TRANSPLANT AMAN RICE VARIETIES
An experiment was conducted at the Agronomy Field Laboratory, Bangladesh Agricultural University, Mymensingh during July to December 2015 to evaluate the effect of row spacing on the performance of three short duration t...