Comparative diagnosis of urinary tract infection (UTI) using urinary nitrite and significant bacteriuria (SBU)
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 4
Abstract
The clinical laboratory diagnosis of urinary tract infection was compared in two hundred (200) midstream urine samples using bacteria culture and urinary nitrite detection technique. The comparative susceptibility of the isolates to common antibiotics was evaluated using completely randomized design (CRD). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for each antibiotics test was evaluated using standard laboratory procedures. Approximately fifty one percent (101/200) of urine samples that were culture yielded significant bacteriuria (SBU) as compared to (32.59%, 65/200) which had positive nitrite detection. Also eighteen percent (18%, 35/200) of the negative nitrite detection test showed evidence of significant bacteriuria. Significant bacteriuria was significantly associated at p<0.05 with culture isolation technique. A total of nine (9) different bacterial isolates were detected in this study. The isolates and their frequency of occurrence were Escherichia coli 30(29.7%), Pseudomonas aeruginosa 15(14.9%), Klebsiella pneumonia 13(12.8%), Enterococcus faecalis and Citrobacterfreundii 10(9.9), Proteus mirabilis 9(8.9), Staphylococcus aureus 8(7.7%), Serretiamarcesens and Streptococcus specie 3(3.0%).The mean total viable count ranged from 31.50±3.15x10 7cfuml-1 to 262.5±1.09x10 8cfuml-1. The antibiotics susceptibility profile reveals a high level of susceptibility of most isolates to Gentamycin(50%), Ciprofloxacin(83%), Tarivid(100%), Augumentin(50%) and Levofloxacin(100%) with mean zone of inhibition ranging from 18.6mm to 20.3mm. However, high resistance profile of hundred percent was observed with nalidixic acid, ampicillin and septrin while reflacin resistant rate was 66.7%. Intermediary susceptibility was observed with streptomycin (50%) and ceporex (66.7%). This study therefore reveals the diagnostic superiority of culture method to urinary nitrite detection technique. In addition, it also reveals Escherichia coli as the most frequently isolated agent of bacteriuria. Furthermore, the study shows a high level of susceptibility of urinary isolates to Gentamycin, Ciprofloxacin Levofloxacin, Augumentin and Tarivid.
Authors and Affiliations
John Anuli S. | Department of General Studies, College of Health Technology, Calabar, Nigeria, Mboto Clement I. | Department of Microbiology, Faculty of Biological Sciences, University of Calabar,P.M.B. 1115, Calabar, Nigeria, Corresponding Authors E-mail: profbaseadeone@gmail.com, Agbo Bassey E. | Department of Microbiology, Faculty of Biological Sciences, University of Calabar,P.M.B. 1115, Calabar, Nigeria
Keywords
Related Articles
- EP ID EP11644
- DOI -
- Views 296
- Downloads 9
Electrocardiographic Profile of Cardiotoxic Plants and Animals
There are numerous plant and animal toxins that can cause electrocardiogram changes, even in patients without history of cardiac pathology. Despite of extensive studies of poisons of plant and animal origin, only few ar...
The level of professional socialization of nursing students of Ilam University of Medical Sciences in 2016
Socialization is a basic and fundamental concept in nursing. There is a great need to evaluate professional socialization of nursing students. The aim of study was determining the factors affecting on the level of profe...
Relationship between Emotional Awareness and Social Creativity in Primary School Students
The present research reviews the relationship between emotional awareness and social creativity. The participants of this research were 234 (120 girls and 114 boys) of the primary school students of the four districts o...
EFFECT OF OCIMUM SANCTUM LINN. IN STRESS INDUCED GASTRIC ULCERS IN RATS
Aims & Objectives: Ocimum sanctum L. popularly known as Tulsi is a medicinal plant that has been used for curing various diseases since ages. In the present study we used leaf extract of Ocimum sanctum for its anti-ulce...
NONLINEAR DYNAMIC ANALYSIS OF VOICE: A NORMATIVE STUDY IN THE INDIAN POPULATION
Background: The aim of this study was to establish normative data for the Indian population using Nonlinear dynamic analysis. In this study, correlation dimension, a measure of nonlinear dynamic analysis was performed f...