Comparative evaluation of antimicrobial efficacy of MTAD, DMSA (Di Mercaptosuccinic acid), sodium hypochlorite (NaOCl) and chlorhexidine against E. faecalis: An ex-vivo study
Journal Title: IP Indian Journal of Conservative and Endodontics - Year 2017, Vol 2, Issue 4
Abstract
Aim: The aim of this study was to compare the anti-microbial efficacy of four root canal irrigants, MTAD, 10% Di Mercaptosuccinic acid (DMSA), 5.25% Sodium Hypochlorite and 2% Chlorhexidine gluconate against Enterococcus Faecalis. Materials & Methods: 75 extracted single rooted teeth with single canal were selected for this study. Conventional access preparations were made and the canals were instrumented 1 mm beyond the apical foramen with K-files up to size 50. The teeth were autoclaved. Pure culture of E. Faecalis was grown in brain heart infusion broth and was set to 4 Mac Farland’s standard. 5 micro-litre of the broth was used to infect each of the root canal. The teeth were then divided into 5 groups (n=15) according to the irrigant delivered; group 1 was irrigated with MTAD, group 2 with 10% DMSA, group 3 with 5.25% Sodium Hypochlorite, group 4 with 2% Chlorhexidine gluconate and group 5 was irrigated with Normal saline (control group). Samples were collected from the root canals, after 5 minutes and 48 hours of irrigation, with help of absorbent paper points and were immediately transferred to test tubes containing brain heart infusion broth and incubated. Occurrence of broth turbidity was indicative of the viable bacteria remaining in the root canal. The test tubes where turbidity occurred were inoculated onto chocolate Agar plates to check for E. Faecalis. Results were analyzed statistically using Chi-Square test and Mann-Whitney test (p<0.05). Results: Viable bacteria were found in all the canals of the control and 10% DMSA group. No viable bacteria were remaining in canals after 5 min of irrigation with MTAD and CHX whereas 67% of the root canals irrigated with NaOCl showed growth. After 48 hours of irrigation only teeth irrigated with CHX were free from viable bacteria.
Authors and Affiliations
Vivek Kapoor, Ruchi Singla, Rajni Kapoor
Keywords
Related Articles
- EP ID EP321239
- DOI -
- Views 67
- Downloads 0
Efficacy of anti-oxidants when used for distinctive time to re-establish bond strength
Introduction The objective was to evaluate the effect of three antioxidants Sodium thiosulphate Sodium ascorbate and Rosemarinic acid with different application times in reversing the bond strength of dentine compromised...
Clinical and radiographic characteristics of the chronic apical periodontitis in senegalese population
Aim This study proposes to describe the epidemiological clinical and radiographic characteristics of Chronic Apical Periodontitis CAP in a population of patients attending Conservative Dentistry clinic of Odontology Depa...
Comparison of the effect of two MMP inhibitors on microtensile bond strength to dentin in class I cavities
Aim To compare the effects of pretreatment with doxycycline and chlorhexidine on microtensile bond strength to dentin in class I cavities restored with composite resinMaterials and Methods Class I cavities were prepared...
Successful endodontic management of permanent maxillary first molar with six root canals by CBCT as a diagnostic aid
The possibility of morphologic variations in the tooth should never be misapprehended and thorough knowledge of their variations is the cue to a successful root canal treatment. Maxillary first molar have been studied fo...
Removal of broken endodontic instrument using Ultrasonic’s and Magnifying loupes– A case report
Separation of endodontic instrument in the middle third of the root canal hinders accessibility to the apical terminus thus compromising cleaning & shaping procedure, which ultimately have a potential impact on the outco...