Abstract

Objective: To investigate the antimicrobial and antioxidant properties of Et2O/MeOH/petrol extract and three isolated compounds from Artemisia kulbadica (A. kulbadica). Methods: The antimicrobial activity was tested by using the disc-diffusion method and determining the minimal inhibitory concentration (MIC) using the agar dilution method against Gram positive and Gram negative bacteria, and fungi. The antioxidant activities of crude extract and tree isolated compounds were evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. Results: The plant extract was showed moderate values DPPH radical scavenging activity (IC50= (422.4 暲 2.4) 毺 g/mL) while it was showed no considerable antimicrobial activity against tested microorganisms. Three isolated compounds tested for the first time, demonstrated antimicrobial and antioxidant activities. Two sesquiterpenes showed higher antimicrobial activity than the flavone while the later compound was better antioxidant than the sesquiterpens. Conclusions: The present study clearly demonstrated that A. kulbadica and some of its isolates each one separately possess antimicrobial or antioxidant properties and may act as potential antioxidant for biological systems susceptible to free radicalmediated reactions.

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