Comparative evaluation of GTR membranes to assess their bacterial activity in vitro
Journal Title: International Journal of Allied Medical Sciences and Clinical Research - Year 2018, Vol 6, Issue 1
Abstract
Background Periodontal regeneration is a reproduction or construction of a lost or injured part in such a way that the architecture and function of the lost or injured tissues are completely restored. Guided tissue regeneration has been used to regain lost periodontal attachment around teeth. Microorganism can adhere to and colonize exposed membranes leading to the development of nidus of infection. Aim of this study is to evaluate adherence of bacteria Porphyromonas gingivalis over Amnion, Chorion and Collagen GTR membrane. Methodology The membranes were placed in tubes containing broth cultures and identical concentrations (1×10 8) of these bacteria at 37ᵒC. At 4, 6,12 and 24 hrs. The bacterial cultures were decanted and the membranes in the tubes were agitated gently, in reduced transfer fluid for 4 times to remove non adherent bacteria. Each tube was sonicated for 30 sec in 10 ml RTF to detach adherent bacteria. The detached adherent bacteria were counted using a Petroff-Hausser chamber. Result P. gingivalis can adhere to Chorion, Amnion and Collagen membranes at interval of 2hrs, 4 hrs and 24 hrs. Conclusion P.gingivalis has higher affinity of adherence towards Chorion as compared to Amnion and Collagen membranes.
Authors and Affiliations
Pallavi Giridhar Sonawane
Keywords
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