Comparative Histochemical Study on Lingual Glands of Some Mammals
Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2019, Vol 18, Issue 2
Abstract
In tongue the, lingual glands secrete saliva containing mucin which forms a protective layer on mucosal surfaces of tongue. Being viscoelastic, mucin is responsible for maintaining lubrication and hydration of the surface. The glands adapt to the necessities of each species. Keeping this in mind we conducted a comparative histochemical study on sublingual glands of five species of mammals for characteristic of composition of salivary secretion.Tissues from all five mammals were stained by alcian blue ph 1.0 before & after active methylation and by aldehyde fuchsin for Highly sulphated acid mucopolysaccharides. For Weakly sulphated acid mucopolysaccharides they were stained by Alcian blue ph 2.5 before and after mild methylation . For Simultaneous demonstration of sulphated and Neutral mucopolysaccharides PAS procedure was applied with alcian blue at ph 1.0 .To look for metachromatic properties Toludine blue at ph 2.0 and 4.4 was used. For simultaneous demonstration of highly and weakly sulphated acid mucopolysaccharides aldehyde fuchsin - alcian blue ph-2.5 sequence was used while for Glycogen and neutral mucopolysaccarides PAS procedure and. PAS phenyl hydrazine were used. On examination at different magnification only specimen from rabbit was found to have the serous glands as purely serous, where as in the rest of animals the serous alveoli were lined by seromucous cells with variable presence of different types of mucopolysacchrides. The presence of moderate amount of weakly sulphated mucopolysacchrides and neutral mucopolysacchrides was found in dog .In albino rat, rabbit and guinea pig highly sulphated mucopolysacchrides were predominant in mucus alveoli followed by weakly sulphated mucopolysaccharide ; but in goat and dog, main mucopolysacchrides were weakly sulphated mucopolysacchrides. Highly sulphated forms were absent in goat and insignificantly present in dog. Except goat , in all animals the same cells in same mucus alveoli , showed the presence of both sulphated and neutral forms.
Authors and Affiliations
K. K. P. Singh, Kumari Sandhya
Keywords
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- EP ID EP465355
- DOI 10.9790/0853-1802045258.
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