COMPARATIVE QUANTITATIVE PHYTOCHEMICAL ANALYSIS OF THE LEAVES OF SENNA ITALICA COLLECTED FROM DIFFERENT AREAS IN LIMPOPO PROVINCE, SOUTH AFRICA
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 2
Abstract
Objective: The current study was aimed at comparative quantitative phytochemical analysis of the leaves of S. italica collected from four districts in Limpopo province, South Africa, in order to establish whether geographical location have an effect on the accumulation of phytochemicals within the leaves of the plant species under study.Methods: The leaves of S. italica were collected from four districts in Limpopo province of South Africa namely; Capricorn, Sekhukhune, Vhembe and Waterberg districts, dried, ground to powder and extracted using different organic solvents. The extracts of the leaf samples from different locations were subjected to quantitative phytochemical analysis for total phenolic content, total tannin content, total flavonoid content and total saponin content using spectrophotometric measurements. The resultant quantities were analysed for statistical differences.Results: The leaf samples of S. italica from the four districts in Limpopo province showed significant differences (*p<0.05) in their phytochemical quantities, with main data expressed as mean±SD. Total phenolic content was in highest amounts in leaf samples from Waterberg district compared to samples from other districts. Total tannin content was in highest amounts in the Vhembe district leaf samples compared to samples from other districts. Total flavonoid content was in highest amounts in the leaf samples from Waterberg district compared to samples from other districts. Total saponin content was in highest amounts in the Vhembe district leaf samples compared to samples from other districts.Conclusion: The findings of the study thus suggest that geographical location has an effect on the accumulation of phytochemicals in the leaves of S. italica.
Authors and Affiliations
S. S. Gololo, N. S. Mapfumari, M. A. Mogale
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