COMPARATIVE STUDIES OF ANTIOXIDANT ACTIVITY FROM THE PETALS OF SELECTED INDIAN MEDICINAL PLANTS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: The present study was to evaluate the in vitro antioxidant activity from the petals of four different Indian medicinal plants (Punica granatum, Hibiscus rosasinensis, Cassia auriculata and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed for flavanoids using standard procedures. The methanol extracts were also evaluated for the antioxidant and free radical scavenging potential by employing different in vitro assays such as reducing power assay, metal chelating activity, hemoglobin-induced linoleic acid peroxidation assay, H2O2 (Hydrogen peroxide), NO (Nitric oxide), Superoxide anion scavenging assay, ABTS (2, 2’-Azino–bi's (3-ethyl benzothiazoline–6–Sulfonic acid) assay and Hydroxyl radical scavenging assays. The total phenolic and flavonoid contents were assessed by Folin-Ciocalteau and aluminium chloride reagents.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavanoids. From the above study, the results indicated that the methanolic extract of Hibiscus rosasinensis petals showed highest radical scavenging activities in all assay techniques at 20 µl/ml and also possessed abundant phenolic (62.4%) and flavanoid contents (73.2%) respectively when compared to other Indian medicinal plants.Conclusion: The results obtained in the present study indicate that the petals of Hibiscus rosasinensis are a potential source of natural antioxidants which may be due to its abundant phenolic and flavonoid contents.Â
Authors and Affiliations
Sumathy Rengarajan, Vijayalakshmi Melanathuru, Deecaraman Munuswamy
Keywords
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