Comparative Studies of EDTA, Sodium Citrate and Aqueous Extract of Triclisia Dictyophylla
Journal Title: Scholars Academic Journal of Biosciences - Year 2013, Vol 1, Issue 2
Abstract
Some anticoagulants used in the laboratory for in vitro studies have some de-merits while others confer some changes on cellular components. Hence, the need to identify and characterize new anticoagulants. Aqueous extract of Triclisia dictyophylla was prepared and compared with EDTA and sodium citrate. 6mls of blood was collected from 200 human volunteers and dispensed 2mls each into EDTA, sodium citrate and 200mg of the extract. Standard methods were used to analyze the samples and the results were compared. The extract showed significant increase (p<0.05) in PCV, Hb, platelet count, ESR and plasma viscosity while WBC showed significant reduction when compared. The refined extract could possess better anticoagulation activity which maybe of importance in the laboratory for in vitro use. Keywords: EDTA, sodium citrate, Triclisia dictyophylla, refined extract
Authors and Affiliations
Anthony C. Ezimah,, Anslem O. Ajugwo,, Felix N. Osuala And, Kevin Aghatise
Keywords
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