Comparative Study of Acid Pretreatment on Saccharification of Sawdust
Journal Title: PARIPEX-Indian Journal of Research - Year 2016, Vol 5, Issue 1
Abstract
Lignocellulosic materials can be utilized for bioethanol production but its recalcitrant nature reduces its large scale utilization. The present study includes the comparative analysis and development of effective pretreatment process to break down sawdust which can releases a better amount of fermentable sugars. Three different acids Viz. H2SO4, HCl and Phenyl acetic acid were used for the pretreatments of sawdust followed by enzymatic hydrolysis with crude and pure form of cellulase. The amount of liberated sugars was measured by DNS method. Acid treatment with H2SO4, HCl and Phenyl acetic acid released 610 μg/mL, 650 μg/mL and 552 μg/mL of sugar respectively. Bioethanol production from saccharified sawdust was carried out by using Saccharomyces cerevisiae. Treatment with 0.1% w/v of pure cellulase enzyme to the acid treated sawdust can produced better amount of ethanol compared to crude cellulase and 0.01% w/v of pure cellulase.
Authors and Affiliations
More Bhikhu, Shah Gaurav
Keywords
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