Comparative study of azelastine and verapamil in the modification of ovalbumin sensitized lung parenchymal tissues of guinea pigs in vitro
Journal Title: Pakistan Journal of Pharmacology - Year 2012, Vol 29, Issue 1
Abstract
Pharmacological prevention of antigen-antibody mediated reaction can be accomplished, at least in principle either to prophylaxis achieved by inhibiting the release of chemical mediators and/or blocking the tissue receptors that served as target of the mediator action. The ability of Azelastine and Verapamil to influence antigen induced contractile responses in isolated sensitized parenchymal tissues of Guinea pig in vitro. Guinea pig (n=10) were sensitized with ovalbumin day 0, day 2, day 21 and keep in the animal house. After twenty one days of ovalbumin sensitization the Guinea pigs were killed by decapitation and exsanguinations and isolated parenchymal tissues challenge with ovalbumin to confirm the sensitization before the study design carried out. Sensitized parenchymal strips with ovalbumin were exposed to different concentration of ovalbumin to calculate the EC50. Each parenchymal strips treated with either Azelastine or Verapamil in an organ bath for 10 minutes and treated with EC50 ovalbumin and contraction recorded by Grass polygraph model 7B. EC50 (n=6) of parenchymal strips was 0.3x10-6+0.16x10-6g/ml and give mean response of contraction (n=6) 9+0.44 millimeter. Azelastine in concentration 10-9 g/ml does not exhibit any inhibitory effects but as the concentration increases to 10-8 g/ml marked inhibition recorded and at concentration 10-7 g/ml completely antagonized the EC50 induced contraction. While the Verapamil does not show any inhibition at concentration 10-10 g/ml and concentration 10--8 g/ml showed complete antagonism. It is concluded that inhibition of ovalbumin induced contraction of sensitized parenchymal tissues of Guinea pig in vitro study is dose dependent and controlled better with Verapamil than Azelastine
Authors and Affiliations
Syed Hasan, Musarrat Sultana, Sheikh Ahmed, Muhammad Salat
Keywords
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