COMPARATIVE STUDY OF HEMOGLOBIN ESTIMATION BY DIFFERENT METHODS
Journal Title: GJRA-Global Journal For Research Analysis - Year 2019, Vol 8, Issue 1
Abstract
Background: Hemoglobin (Hb) estimation is one of the most frequently ordered investigations. Estimation of exact levels is important to stratify the grade of anemia and subsequently direct the necessary treatment. Conventionally, Hb has been estimated using colorimetric method, which is time tested and recommended by the WHO. Now-a-days, the automated method is also becoming a popular method in many hospitals. However, there are not many studies assessing the accuracy of the automated method over the gold standard. Materials and methods: We retrospectively analyzed hemoglobin values in 180 adult patient-samples (18 per batch in 10 batches). Hemolyzed samples were excluded from the study. Blood samples were drawn in vials having K3 EDTA anticoagulants. After a proper mixing, hemoglobin was estimated by automated Sysmex XS-800i. Parallel estimation for Hb content was done manually by spectrophotometer 4010. Results: Patients ranged from ages 20 to 40 years (M:F=102:78). The lowest value recorded by Sysmex XS-800i was 5.8 while the highest value recorded was 18.6 gm%. The mean hemoglobin concentration on Sysmex XS-800i was 12.89. The lowest, highest and mean values recorded by the cyanmethemoglobin method on photometer were 5, 18 and 13.49 gm% respectively. This showed a mean difference of 0.597 and with signicant p-value of <0.001. Conclusion: The lowest values of Hb were similar in both the methods but the mean as well as the highest values differed signicantly. Our study found an accuracy of 95.57% with the 5 part analyzer when compared to the gold standard colorimetric method.
Authors and Affiliations
Dr. Priya Singh, Prof. (Dr. ) Sharique Ahmad, Dr. Sudarshana Gogoi, Prof. (Dr. ) A N Srivastava
A STUDY ON PERSONAL VALUES AND CRITICAL THINKING OF B.ED STUDENTS
The intention of the research is to study the personal values and critical thinking of B.Ed students. In order to study this problem, the survey method was used to collect the data. The population of the present investig...
TREATMENT OUTCOME OF EXTERNAL BEAM RADIOTHERAPY (EBRT) WITH INTRACAVITARY RADIOTHERAPY (ICRT) IN CA CERVIX : AN INSTITUTIONAL EXPERIENCE
Introduction : Ca Cervix is the second most common malignancy in Indian women. Most of the cases present in advanced stage. Radiotherapy is an important modality of treatment. Combination of EBRT and ICRT is an effective...
Umbilical Pilonidal Sinus –A Rare Case Report
Pilonidal sinus is a common disease of sacrococcygeal region involving natal cleft. However, it is also observed in the periumbilical area. There are only a few reports about umbilical pilonidal sinus in the literature....
GAUGING PERFORMANCE OF MUTUAL FUNDS BEING SOLD THROUGH RETAIL BANKING IN INDIA
A Mutual Fund is a corporation and the fund manager’s interest is to professionally manage the funds provided by the investors and provide a return on them after deducting reasonable management fees. Main objective of th...
STOCK MARKET REACTION TO THE BUYBACK ANNOUNCEMENT -- AN ABNORMAL RETURNS ANALYSIS
The main objective of the paper is to analyze and interpret the impact of the buyback of equity announcement on the market price in the Indian context. The sample size of the paper consists of 64 initial buyback programs...