Comparing Effects of Intraventricular Hypertonic Saline and Magnesium Sulfate Application on Diffuse Brain Injury in Rats
Journal Title: Türk Yoğun Bakım Dergisi - Year 2017, Vol 15, Issue 1
Abstract
Objective: Diffuse brain injury is one of the most common issues encountered in, patients with trauma and it leads to morbidity and mortality via increased intracranial pressure. The aim of the study was to compare the effects of magnesium sulfate and hypertonic saline on diffuse brain injury in rats. Materials and Methods: In this study 18 male Sprague-Dawley rats weighing 250-300 g were used. The rats were randomly divided into trauma (control), trauma+magnesium, and trauma+hypertonic saline groups. Traumatic brain injury was induced by modified Feeney head trauma model. A single dose of 10 µL isotonic saline, magnesium sulphate and hypertonic saline were applied intraventricularly to the control, magnesium, and hypertonic saline groups, respectively. Rats were decapitated 24 hours after the head trauma. Their brains were dissected immediately and stored with dry ice at -80 °C for histopathological experiments. Results: The number of damaged neurons were significantly higher in both control and hypertonic saline groups (p=0.001, p=0.008). However, the number of damaged neurons did not show significant difference between hypertonic saline and control groups, it was significantly lower in magnesium group (p<0.05). Conclusion: In this study, intraventricular magnesium application is found effective in reducing the number of the damaged neurons in rat traumatic brain injury model. These results suggest that magnesium usage may be evaluated for the treatment of patients with traumatic brain injury in further prospective studies.
Authors and Affiliations
Nurcan Kızılcık, Erhan Çelikoğlu, Taha Keleştemur, Özge Köner, Merih iş, Sevgi Bilgen, Ayçiçek Çeçen, Bayram Yılmaz
Evaluation of Obstetric Patients Who Were Admitted to the Intensive Care Unit
Objective: To evaluate all obstetric patients admitted to intensive care unit (ICU). Materials and Methods: Ninety-four obstetric patients were evaluated for age, mortality, preexisting disorders, the reason for admissio...
Posterior Reversible Encephalopathy Syndrome: Two Case Reports
Posterior reversible encephalopathy (PRES) syndrome is a clinical-radiological condition characterized by headache, changes in consciousness, epilepsy and visual disturbances, and usually occurs in the posterior parietal...
Evaluation of Gram-Negative Bacilli Isolated from Patients in Intensive Care Units
Objective: A significant proportion of nosocomial infections resulting in significant mortality and morbidity for hospitalized patients are due to the patients in intensive care units (ICU). For this reason, the identifi...
Effect of Noise on Hospitalized Patient’s Night’s Sleep and Vital Signs in Intensive Care Unit
Objective: Noise is defined as unwanted sound causing discomfort. The aim of this study was to determine the effect of noise on sleep in intensive care patients and to examine its impact on vital signs. Materials and Met...
Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Patients Admitted to the Intensive Care Unit of Hatay State Hospital
Objective: In this study, it was aimed to determine vancomycin resistance mechanisms, virulence genes and clonal relationships of 23 vancomycin-resistant Enterococcus faecium (VREfae) strains isolated from patients admit...