Comparing genetic diversity and population structure of common beans grown in Kyrgyzstan using microsatellites
Journal Title: Scientific Journal of Crop Science - Year 2012, Vol 1, Issue 4
Abstract
Common bean (Phaseolus vulgaris L.) is an important export crop in Kyrgyzstan. The aim of this study was to assess the extent of genetic diversity, determine the population structure, and relate to the main gene pools grown in Kyrgyzstan. Twenty-eight common bean accessions (including five Kyrgyz cultivars, and main references from the Mesoamerica and South America) were evaluated with microsatellites. Nine polymorphic microsatellites were used to estimate genetic diversity and heterozygosity. The number of alleles per microsatellite locus ranged from 2 to 4 and there were a total of 24 alleles. The observed heterozygosity of each accession over all loci ranged from 0 to 1.11 (with an average of 0.05), while the expected average heterozygosity was 0.08, which could reflect the self-pollinating breeding behavior of common beans. The analysis of molecular variance further revealed that 94.71% of the total variation was accounted by differences among accessions (Fst =0.947; p<0.001). Cluster analysis grouped accessions in two gene pools: 16 belong to the Andean and 12 to the Mesoamerican gene pool. The microsatellites separated accessions in Mesoamerican gene pool from Durango and Jalisco races, which were grouped together. We also observed that the most divergent accessions were the Kyrgyz cultivars, which may be related to the Mesoamerican races. Andean accessions were less diverse than Mesoamerican accessions in this study. This research confirms the ability of microsatellites to differentiate common bean accessions, even using a small sample size, and to be able to assign modern cultivars to their gene pools or races.
Authors and Affiliations
S. Hegay*| Department of Agronomy, Kyrgyz National Agrarian University, Kyrgyzstan., M. Geleta| Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Sweden., T. Bryngelsson| Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Sweden., L. Gustavsson| Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Sweden., H. P. Hovmalm| Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Sweden., R. Ortiz| Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Sweden.
Keywords
Related Articles
- EP ID EP1000
- DOI -
- Views 552
- Downloads 39
Identification of Ixodide ticks of cattle in and around Hararamaya district, Eastern Ethiopia
We conducted a survey to determine the prevalence of Ixodide ticks, their predilection sites and relation to breed, sex, and age group of animals. A total of 560 animals were examined of which 186 (33.21%) found infested...
Effect of iron nano chelated fertilizers foliar application on three wheat cultivars in Khorramabad climatic conditions
In order to investigate the effect of iron nano chelatedfertilizers foliar application on three wheat cultivars, an experiment wasarranged based on randomize complete block designe in three replications duringthe 2011-20...
Phytochemical screening and physical constant evaluation of Ficus Abutilifolia Miq. (Moraceae) leaves, stembarks and roots for quality control
Ficus abutilifolia has been used to treat various ailments in Nigeria especially in the Northern and Southern parts, where the plant’s aqueous extracts has been use to treat typhoid fever, syphilis, and amoebic dysentery...
Pre-scaling-up of malt barley technologies in bale and west Arsi zones, Southeastern Oromia, Ethiopia: The experience of public-private partnership
The paper presents the out-put, progress and success of pre-scaling up activities of improved malt barley technologies in Sinana, Goba, Dinsho and Gassera Districts of Bale Zone, and Adaba and Dodola Districts of West Ar...
Seed quality of Lentil (Lens culinaris L.) as affected by different containers and storage periods
To investigate the effect of different containers and storage periods on the seed quality of lentil seed, the present study was carried out at Department of Agronomy, Hajee Mohammad Danesh Science and Technology Universi...