Comparism of Presence of Foot and Mouth Disease Antibodies using Liquid Phase Blocking Elisa in Market and Ranch Cattle in Umuahia Abia State, Nigeria
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 3
Abstract
A serological study was conducted to ascertain the presence of Foot and Mouth Disease (FMD) in cattle in three cattle farms and two cattle markets in Abia State. Eighty-eight sera were screened for FMD antibodies using the PrioCHECK® 3ABC NSP ELISA kit. The assay procedures were performed in accordance to the kit manufacturers’ instructions. Test plate was read using the spectrophotometer (ELISA reader) at 450nanometer. The percentage inhibition (PI) of the optical density (OD) from each sample was calculated using the formula: PI=100-[OD450test sample /OD450max] ×100. Sample was said to be negative when PI is <70 and positive when PI is ≥ 70 as indicated by the manufacturer. Higher sero-prevalence was recorded in cattle samples from the market with a sero-prevalence rate (SPR) of 78.3%. There was a lower SPR of 40.5% from farm cattle; the overall SPR was 60.2%. There was no significant difference in seropositivity between farm and market cattle P > 0.05 using student t-test. The result confirmed the presence of FMD which stands as a production constraint to small cattle holders in the area; the epidemiological importance of cattle markets in FMD perpetuation base of high SPR in market animals.
Authors and Affiliations
Tchokote E. Y.
Keywords
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