Comparison between tuberculin test results and ELISA for diagnosis of bovine tuberculosis
Journal Title: Journal of Veterinary Medical Research - Year 2008, Vol 17, Issue 1
Abstract
A total of 300 cross-bred dairy cattle in Beni-Suef and El-Fayoum Governorates were screened for bovine tuberculosis using single intradermal (SID) cervical tuberculin test. 18 out of 300 (6%) tested cattle were found tuberculin positive. Blood samples from the positive reactors were tested by ELISA. ELISA plates were coated by either bovine purified protein derivative (PPD) or short term culture filtrate (ST-CF) antigens. The test sensitivity was compared at different serum dilutions. At serum dilution of 1/40, all of the 18 tuberculin positive samples, (100%), were ELISA positive using both ST-CF and PPD antigens, but at 1/80 dilution, 13 (72.22%) and 12 (66.66%) samples; at 1/160, 11 (61.11%) and 11 (61.11%) and finally at 1/320, 10 (55.55%) and 9 (50%) were ELISA positive on using ST-CF and PPD as a coating antigens respectively
Authors and Affiliations
F. R. El-Seedy1; Sohair S. Elyas2; W. H. Hassan1; E. A. Nasr3 1Department of Bacteriology Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt 2Animal Health Research Institute Dokki, Gizza, Egypt 3Veterinary Serum and Vaccine Research Institute Abbassia, Cairo, Egypt
Keywords
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