Comparison of analytical sensitivity of HIV diagnostic kits using dilutional panel
Journal Title: International Journal of Biomedical and Advance Research - Year 2016, Vol 7, Issue 4
Abstract
An array of tests have been developed over a period of time for screening and diagnosis of HIV infection which include tests for detection of antigen and antibody , viral nucleic acid and the virus itself. Amongst the available tests, serodiagnosis by ELISA is widely being used. It offers a relatively inexpensive procedure with ease of performance and ability to effectively screen a large number of specimens. Our current study is designed to compare the performance of two different HIV diagnostic kits having different coated antigens using HIV dilutional panel. Kit X had synthetic peptides (3rd generation), and the Kit Y had recombinant antigens (2nd generation) coated on the microtitre plates. 10 HIV-1 plasma panel members which were collected from various blood banks were used for comparison of their performance. The plasma samples were diluted in human plasma (base matrix) negative for anti HIV, anti HCV, HBsAg, VDRL and HTLV I/II. Serial dilutions in the range of 10 to10,000 fold for each sample were used for this study. These kits were compared in terms of antibody titre which is defined as the dilution at which the sample OD to cut off (S/Co) value is 1. It was seen that Kit X is more sensitive as compared to Kit Y for all the HIV panel members. This indicates that Kit X had better analytical sensitivity performance as compared to Kit Y since the S/Co ~ 1.0 of Kit X is at a higher dilution (1:1000) than of Kit Y (1:100).Thus this indicates that dilutional panel can help the manufacturers to design a diagnostic kit which is able to detect the infection at an early stage of disease. It was seen that the S/Co ~ 1.0 of kit X at a higher dilution.
Authors and Affiliations
G. Bindra, R. Chhabra, S. Sharma, PSC Anand, B. Bhushan
Keywords
Related Articles
- EP ID EP364303
- DOI 10.7439/ijbar.v7i4.3138
- Views 68
- Downloads 0
Improved Spectrophotometric Pharmacopoeial Method for accurate Quantitation of Thiomersal content in Haemophilus influenzae type b (Hib)-TT Conjugate Vaccine
Vaccines remain one of the most effective ways to prevent infectious diseases globally. Accurate measurement of thiomersal content is an important parameter to ensure safety of vaccines though these vaccines are less in...
Clinical Presentation, Risk Assessment and Management of Polycystic Ovary Syndrome (PCOS)
Polycystic ovary syndrome (PCOS) is a complex and heterogeneous disorder characterized by hyperandrogenemia, hyperinsulinemia, insulin resistance, and chronic anovulation. It is the most common endocrine disorder in wome...
Intrahepatic pseudocyst complicating acute pancreatitis: A rare case report
Intrahepatic pseudocysts are an extremely rare complication following acute pancreatitis with only a few cases previously described in literature. The paucity of experience and relevant literature on this condition leads...
Formulation and evaluation of rapid dissolving films of Pravastatin sodium
The concept of Fast dissolving drug delivery system offers a solution for those patients having difficulty in swallowing tablets/capsules etc. This work investigated the possibility of developing Pravastatin sodium rapid...
Study of serum lipid profile in subclinical hypothyroidism
Objective: It has been known that overt hypothyroidism is associated with hyperlipidemia, replacement therapy with Levothyroxine significantly reverses lipid metabolism abnormalities. But no clear consensus has been esta...