Comparison of Anti-bacterial Activity of Different Concentrations of Betel Leaves (Piper betle) and Curry Leaves (Murraya koenigii) Extract on Streptococcus mutans - An in vitro Study
Journal Title: International Journal of Ayurveda and Pharmaceutical Chemistry - Year 2017, Vol 0, Issue 0
Abstract
Background: The nature has answer to cure every disease; medicinal plants are richest bioresource of traditional systems of medicine. In Ayurvedic medicine the Betel leaves are useful for various systemic diseases. The practice in India is to chew Betel leaves alone or with other spices, which acts as “breath fresheners” and helps in preventing halitosis. Curry leaves are used as taste maker in Indian Cooking. Aim: To compare antibacterial activity of Betel leaves and Curry leaves extract on Streptococcus mutans using different concentrations. Methodology: Extracts of Curry leaves and Betel leaves were prepared by cold maceration technique using both alcohol as well as aqueous solutions. Different dilutions of distilled water were prepared viz., 20%, 40%, 60%, 80% and 100% of aqueous extracts, while 100% alcohol was used to prepare dilutions of alcoholic extracts. The dilutions of alcoholic extracts were prepared in two concentrations:-50% and 100%. Ditch plate method was used to measure the effects of extracts on growth of Streptococcus mutans. Inhibition zones were measured with use of Vernier caliper and compared. Experiment was repeated thrice and means were calculated. Results: Extracts of curry leaves and betel leaves showed significant antimicrobial activity against Streptococcus mutans. It was found that 100% alcoholic extract of Betel leaves is most effective with maximum zone of inhibition while 20% aqueous extract of curry leaves is least effective. Piper betle extract has more antibacterial activity than curry leaves extract. Conclusion: Commonly using Curry leaves and Betel leaves extract can be used to prepare mouthwashes which are effective against cariogenic bacteria. These mouthwashes might acceptable, an easily accessible and provide cheaper alternative to commercially available allopathic mouthwashes leading to achievement of affordable quality health care
Authors and Affiliations
Abhishek A. Talathi, Anil V. Ankola
Keywords
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