Comparison of bloody pap smear fixation by Carnoy’s and fixator spray in samples from women with abnormal uterine bleeding
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 5
Abstract
Abnormal uterine bleeding is a serious problem among women of late reproductive age. Cervical cancer is one of the reasons of AUB and Pap smear is the best way to diagnose it. Blood has negative effects on Pap smear especially in AUB with great blood. One of the effective solutions for lysing RBC is Carnoy’s. This study aims to compare two methods of fixation in bloody Pap smear by Carnoy’s and normal spray in samples from women with AUB. This study was done on 204 bloody Pap smear from 102 women with AUB that referred to Zeinabieh and Faghihi hospitals of Shiraz University of Medical Sciences from2012-2013. After observing uterine bleeding in each subject, two samples were provided using usual Pap smear method; one of the slides was fixed by normal spray and the other slide fixed by Carnoy’s solution for20 minutes. After staining (Papanicolaou method), two pathologists performed a double-blind trial to analyze them. Data analysis was done using SPSS. 82 samples did not have cell adequacy. Squamous cells in Carnoy’s-fixed slides were more than spray-fixed ones (p=0.024). The decrease in the number of RBC on slides and the increase in the clarity of slides in Carnoy’s-fixed samples were more than sprayfixed slides (p=0.001), while identifying inflammatory cells(p=0.832) and microbial factors (p=1) in both methods showed no significant difference. Carnoy’s solution can be used as an effective fixative in bloody Pap smear from women with abnormal uterine bleeding.
Authors and Affiliations
Keshavarz Najmeh| Department of Midwifery, Fatemeh College of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran, Abdali Khadijeh| Department of Midwifery, Fatemeh College of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran, Corresponding Email: abdali_kh@yahoo.com, Akbarzadeh Mojgan| Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, Amooei Sedighe| Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, Tabatabaei Sayyed Hamidreza| Department of Epidemiology, Health and Nutrition Faculty, Shiraz University of Medical Sciences, Shiraz, Iran, Dowran Paria| Department of Midwifery, Fatemeh College of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran
Keywords
Related Articles
- EP ID EP11705
- DOI -
- Views 283
- Downloads 10
Effect of Socio-Demographic and Clinical Characteristics on Physical Activity of Pregnant Women at Referral Hospital in Riyadh, KSA
Objective: The objective of this study was to investigate the effects of socio demographic and clinical characteristics of pregnant women on their physical activity. Methods: An observational quantitative cross sectiona...
Relationship between sedative prescribed to children before surgery and parental anxiety at Shahid Chamran Hospital, Shirazin2015
The researcher’s observations at work indicated that many parents will experienceanxious behaviors (cry and so on) while their children are prescribed sedative. The present study was carried out in order to determine...
Assessing the psychometric questioner for students rating teachers
Educational institutions use different ways to evaluate their teachers. Asking students to rate their teachers is common practice. The purpose of this research was to examine the reliability of the instruments used to e...
COMPARISON OF HEMATOLOGICAL PARAMETERS BETWEEN PLASMODIUM FALCIPARUM, PLASMODIUM VIVAX AND CONTROL GROUPز
Aims: Malaria, a morbid disease of Tropical countries, may harmful if it cannot be diagnosed at its early phase, by observing the changes in hematological parameters. Our aim was to compare the hematological parameters...
Social media as a beneficial tool to support preterm infants and parents
Home care, continuous follow-up, and endless post discharge support play vital role in preventing the emergence of mental and physical disabilities and death among preterm infants. Providing necessary care and support f...