Comparison of Classical Conventional Tests to XPERT MTB / RIF in the Diagnosis of Tuberculosis in People Living With HIV in Kinshasa
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 4
Abstract
Background: In countries with a high prevalence of HIV infection, diagnosis of TB / HIV co-infection with low-sensitivity Ziehl is a difficult problem because of lesions that are paucibacillary. Objectives: To compare the Xpert® MTB / RIF test with conventional tests and to determine the level of resistance to rifampicin. Methods: Sputum was tested at Gen-Xpert® MTB / RIF and compared to smear microscopy and culture (Standard Gold). The resistance to rifampicin was determined in comparison with results obtained by the ratio technique on Löwenstein-Jensen. Results: Xpert®MTB/ RIF detected M. tuberculosis in 42 samples (60%), whereas, the cultures showed a positivity rate of 54.3%; 38 strains of Mycobacterium tuberculosis were isolated. The positivity rate was 44.3% at Auramine and 25.7% at Ziehl. The rate of resistance to rifampicin was 10.5% at Xpert® and 7.9% with the ratio technique. The absolute difference between Xpert®-Ziehl was 34.3%; 15.7% for Xpert®-Auramine; 5.7 % for Xpert-Cultures:; 28.6 % for Cultures-Ziehl; 10 % for Cultures-Auramine and 18.6 % for Auramine- Ziehl: Gen-XPERT is 2.3 times better than Ziehl, 1.4 times more potent than Auramine and 1.1 times better than the culture. Conclusions: The Xpert®MTB / RIF test is more sensitive than conventional tests and at the same time give information on resistance to Rifampicin in less than 2 hours.
Authors and Affiliations
Kabedi Bajani MJ, Kayembe Ntumba JM, Kashongwe Munogolo Z, Bisuta Fueza S, Mbaya Kalumba P, Taba Kalulu M, Muyembe Tamfum JJ
Keywords
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- EP ID EP546690
- DOI 10.21276/ijmrp.2017.3.4.019
- Views 67
- Downloads 0
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