Comparison of Commercial and noncommercial Medium on Sperm Motility after Freezing
Journal Title: International Research Journal of Applied and Basic Sciences - Year 2013, Vol 7, Issue 12
Abstract
In general, human sperm is preserved in temperature range under -20°C. Currently, survival rate of the post-thaw sperm is around 50-60%. Although sperm cryopreservation medium including proteins and lipids derived from animals, for example egg yolk as commonly used, the components of proteins and lipids derived from animals are not clear. Therefore, synthetic and plant derived medium was used for the study. In the present study, post-thaw motility frozen with medium including long-chain alcohol and lipids was examined to develop cryopreservation medium for human sperm.During post-cool evaluation of the various extenders no differences were found in the motility between extenders. This observation was anticipated, as there are canine semen extenders commercially available void of egg yolk. An unexpected result was the significant 11% decrease of normal morphology in the 0% egg yolk concentration extender after 3 hours of cooling at 5°C with no significant change in the remaining extenders.
Authors and Affiliations
Azita Azarpoor| Master of Azad University Science and Research, Department of Molecular Cell Biology, A. T. Sarami| gynaecologist,subspecialty in infertility, ivf a laparoscopic surgery,sarem cell research, centersaremwomenshospital,Tehran , Iran, MaarefatGhaffari Novin| MD,Phd
Keywords
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