Comparison of conventional methods with gene xpert mtb/rif assay for rapid detection of mycobacterium tuberculosis and rifampicin resistance in extra-pulmonary samples
Journal Title: International Journal of Medical Research and Review - Year 2016, Vol 4, Issue 2
Abstract
Introduction: Tuberculosis (TB) is a major health problem in India. The World Health Organization has recently in 2010 endorsed the Gene Xpert MTB/RIF assay for rapid detection of smear negative and multidrug resistance tuberculosis and more recently for extra pulmonary tuberculosis. Objectives: Evaluation of role of Cartridge based nucleic acid amplification test (CBNAAT) in extra-pulmonary tuberculosis (EPTB) in comparison with Ziehl Neelsen (ZN) staining and evaluating rifampicin resistance with the same test. Materials & Methods: Extra-pulmonary samples, including pleural fluid, pus, CSF, lymph tissue & others were divided in 2 parts: one for MTB/RIF assay & other for ZN staining. Both were then compared. Results: A total of 300 extra pulmonary samples were processed in this study, which included 103 pleural fluids, 81 pus, 45 CSF, 35 Lymph node tissue, 20 ascitic fluids and 16 synovial fluid. Out of these 37% (111) patients were Gene Xpert MTB/RIF Assay positive and 36 % (40 out of 111) were ZN smear positive. M.tuberculosis was detected in 56.7% pus samples, 23.3% pleural fluid samples, 54.2% lymph node samples, 33.3 % CSF samples, 20% ascitic fluid samples and 18.7% synovial fluid samples. In this study, we found that Gene Xpert MTB/RIF assay is a rapid method for diagnosis of EPTB as compared to conventional methods along with advantage of detecting Rifampicin resistance. Conclusion: Because of its simplicity, rapidity and sensitivity, this seems to be a very novel tool for diagnosis of extra pulmonary tuberculosis from clinical samples and that it should be researched more thoroughly.
Authors and Affiliations
Sanjay Avashia, Deepak Bansal, Kunal Ahuja, Vijay Agrawal
Keywords
Related Articles
- EP ID EP227654
- DOI 10.17511
- Views 165
- Downloads 0
Oral manifestations of type 2 diabetes mellitus of Bhopal city: an observational prospective study
Introduction: Type 2 diabetes mellitus involves a variety of dysfunctions bringing about a few delicate tissue variations from the norm in the oral cavity. These incorporate gingivitis periodontitis, salivary dysfunction...
Use of cutaneous silent period parameters as a diagnostic indicator in patients with restless legs syndrome-a study in south Indian population
Objective: The objectives of this study were to investigate cutaneous-silent- period (CSP) parameters- CSP latency and CSP duration in patients with ‘Restless legs Syndrome’ and compare it with that of normal healthy con...
Histopathological and cytological spectrum of lymphadenopathy: a window to preliminary diagnosis
Introduction: Lymphadenopathy is a common clinical presentation of patients attending outdoor patient departments. The etiology varies with a wide spectrum ranging from inflammatory causes on one end to malignant on the...
Effect of exposure of cotton dust on pulmonary function tests in female workers of spinning mill at Malegaon, Maharashtra
Background: Textile industry is the only industry to have employed women workers since long time. There are many respiratory problems develop in the women workers. Aims and Objective: The aim of this study was to assess...
Spontaneous cervical emphysema: a challenge in establishing a plausible etiology
Spontaneous cervical emphysema occurs in the absence of previous disorders or intiating factors and often poses a challenge in establishing an etiology at presentation. We report a 23 year old lady who presented with ext...