Comparison of direct versus Friedewald methods for determing LDL cholesterol levels in the horse
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 2
Abstract
Objective. To compare the Direct Method vs the Precipitation Friedewald Method for determing LDL cholesterol levels in the horse, specie with HDL pattern. Materials and methods. Blood Samples of 200 overnight fasted horses of different ages, breed and sex were obtained. Blood levels of LDL cholesterol were determined first by the Direct method and then by the precipitation Friedewald method. The results were analyzed using (one way ANOVA) and compared (F test <0.05). Results. There were no differences for determing LDL cholesterol levels between the Direct Method vs Friedewald Method (mean: 27.9 vs 29 mg/dl, minimal: 14 vs 11.2 mg/dl, maximal: 51 vs 54.2 mg/dl, range: 37 vs 43 mg/dl, and standard deviation: 6.4 vs 7.0 mg/dl respectively) Conclusion. According to these results both methods are recommended for determing LDL cholesterol levels in species with HDL pattern.
Authors and Affiliations
José Osório O, Ph. D *| Universidad de Caldas, Departamento de Ciencias Básicas de la Salud, Laboratorio de Bioquímica Clínica y Patología Molecular.Correspondencia: jose.osorio_o@ucaldas.edu.co., Luis Uribe-Velázquez, Ph. D| Universidad de Caldas, Departamento de Salud Animal.
Keywords
Related Articles
- EP ID EP3204
- DOI -
- Views 387
- Downloads 18
APPLICATIONS OF FLOW CYTOMETRY IN MICROBIOLOGY VETERINARY SCIENCE AND AGRICULTURE
Flow cytometry, an automated, multiparametric and quantitative method, analyzes the scattering and fluorescence signals produced as series of single cell pass through a laser beam. The potential of the new methods of ana...
Random regression models for milk, fat and protein in Colombian Buffaloes
Objective. Covariance functions for additive genetic and permanent environmental effects and, subsequently, genetic parameters for test-day milk (MY), fat (FY) protein (PY) yields and mozzarella cheese (MP) in buffaloes...
Performance of growing swines fed with a diet containing rice subproducts
Objective. To evaluate the performance of swines fed with rice sub products during the growing fase. Materials and methods. Forty hybrid piglets of 31.26 ± 4.62 kg, were allotted to 5 groups an experimental randomized bl...
Seroprevalence of in canines and humans Leptospira spp in three neighborhoods of Tunja, Colombia
Objective. To establish the seroprevalence of Leptospira spp in the canine and human populations of slum neighborhoods in the city of Tunja. Materials and methods. Sixty-one canine serums were obtsined in slum neighborho...
Cytological and microbiological evaluation of uterine washing of bovine with reproductive problems (preliminary study)
Objective. Determination of the cytology and microbiology characteristics using low volume uterine lavage in dairy cows with reproductive problems in two herds on the cundiboyacence plateau. Materials and methods. 21 ute...