Comparison of direct versus Friedewald methods for determing LDL cholesterol levels in the horse
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 2
Abstract
Objective. To compare the Direct Method vs the Precipitation Friedewald Method for determing LDL cholesterol levels in the horse, specie with HDL pattern. Materials and methods. Blood Samples of 200 overnight fasted horses of different ages, breed and sex were obtained. Blood levels of LDL cholesterol were determined first by the Direct method and then by the precipitation Friedewald method. The results were analyzed using (one way ANOVA) and compared (F test <0.05). Results. There were no differences for determing LDL cholesterol levels between the Direct Method vs Friedewald Method (mean: 27.9 vs 29 mg/dl, minimal: 14 vs 11.2 mg/dl, maximal: 51 vs 54.2 mg/dl, range: 37 vs 43 mg/dl, and standard deviation: 6.4 vs 7.0 mg/dl respectively) Conclusion. According to these results both methods are recommended for determing LDL cholesterol levels in species with HDL pattern.
Authors and Affiliations
José Osório O, Ph. D *| Universidad de Caldas, Departamento de Ciencias Básicas de la Salud, Laboratorio de Bioquímica Clínica y Patología Molecular.Correspondencia: jose.osorio_o@ucaldas.edu.co., Luis Uribe-Velázquez, Ph. D| Universidad de Caldas, Departamento de Salud Animal.
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