Comparison of direct versus Friedewald methods for determing LDL cholesterol levels in the horse
Journal Title: Revista MVZ Cordoba - Year 2011, Vol 16, Issue 2
Abstract
Objective. To compare the Direct Method vs the Precipitation Friedewald Method for determing LDL cholesterol levels in the horse, specie with HDL pattern. Materials and methods. Blood Samples of 200 overnight fasted horses of different ages, breed and sex were obtained. Blood levels of LDL cholesterol were determined first by the Direct method and then by the precipitation Friedewald method. The results were analyzed using (one way ANOVA) and compared (F test <0.05). Results. There were no differences for determing LDL cholesterol levels between the Direct Method vs Friedewald Method (mean: 27.9 vs 29 mg/dl, minimal: 14 vs 11.2 mg/dl, maximal: 51 vs 54.2 mg/dl, range: 37 vs 43 mg/dl, and standard deviation: 6.4 vs 7.0 mg/dl respectively) Conclusion. According to these results both methods are recommended for determing LDL cholesterol levels in species with HDL pattern.
Authors and Affiliations
José Osório O, Ph. D *| Universidad de Caldas, Departamento de Ciencias Básicas de la Salud, Laboratorio de Bioquímica Clínica y Patología Molecular.Correspondencia: jose.osorio_o@ucaldas.edu.co., Luis Uribe-Velázquez, Ph. D| Universidad de Caldas, Departamento de Salud Animal.
Clinical and histopathological study of the phototoxic dermatitis in Zebu calves in grazing of Brachiaria decumbens
The objective was to study the clinical and histopathological aspects of Phototoxic Dermatitis Secondary (PDS), secondary to ingestion of Brachiaria decumbens in Zebu calves (Bos indicus) department of Cordoba, Colombia.
Fecundity and fertility of Macrobrachium amazonicum (Héller 1862) (Decápoda, Palaemonidae) of Colombian Piedemonte Llanero
Objective. To determine the fecundity and fertility of Macrobrachium amazonicum (freshwater shrimp) of the Piedemonte llanero. Materials and methods. The animals were caught at a fish farm, in the municipality of Restrep...
IMPORTANCE OF THE USE OF DIFFERENT DIGESTIBILITY TECHNIQUES IN PIG NUTRITION AND FOOD FORMULATION
The nutritional value of a meal for pigs could be expressed using a digestibility coefficient whereas the type of digestibility may be assessed by the point of origin of the sample: 1) ileal Digestibility (IDI), attempts...
DETERMINATION of Helicobacter spp., FROM GASTRIC ULCERS IN HORSES
Objective. To determine the presence of bacteria type Helicobacter spp., from gastric ulcers in horses. Materials and methods. Fourty horses, 28 males and 12 females (4-17, years) were included. The frequency of presenta...
DESIGNING AN ASSESMENT TOOL OF ENVIROMENTAL PERFORMANCE INTO FISHING FARMS
With the objective of evaluating the environmental performance of the fish farms facilities, it was designed and it applied a tool based on the check lists, which was elaborated of agreement with the recommendations give...