Comparison of dye-based proteinuria estimation methods with immunoturbidimetric microalbuminuria estimation, in screening for diabetic
Journal Title: International Journal of Clinical Biochemistry and Research - Year 2018, Vol 5, Issue 3
Abstract
We compared the performance of Coomassie Brilliant Blue CBB and Ponceau S dyes based proteinuria estimation methods with immunoturbidimetry microalbumin estimation to develop new cutoffs for proteinuria which would help us detect diabetic nephropathy patients with better sensitivity using the above mentioned dye based methods These proteinuria testing methods are easy to adapt in smaller laboratories typically associated with peripheral government health centersUrine sample was collected from 158 diabetic individuals and tested simultaneously for Urine Albumin Creatinine ratio UACR using immunoturbidimetry and Urine Protein Creatinine Ratio UPCR using Ponceau S method and CBB method The Ponceau S method gives good correlation with immunoturbidimetry for screening of diabetic nephropathy at UPCR cutoff of 50mgg creatinine The CBB method gives higher false positives The overall agreement of Ponceau S and CBB with immunotubidimetry is maximum at cut off 50mgg ie 91 and 84 respectivelyIn conclusion proteinuria estimation with Ponceau S method with UPCR 50mgg creatinine may be used for routine screening of diabetic patients to diagnose microalbuminuriaKeywords Diabetic nephropathy screening Proteinuria Microalbuminuria Ponceau S
Authors and Affiliations
Lalithambigai Arumugasamy
Keywords
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- EP ID EP475181
- DOI 10.18231/2394-6377.2018.0078
- Views 68
- Downloads 0
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