Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods
Journal Title: The European Research Journal - Year 2019, Vol 5, Issue 1
Abstract
Objectives: Liver transplantation is increasingly being used in the treatment of end-stage liver disease. Ischemia-reperfusion injury is one of the major problems encountered in transplantation. In this study, we aimed to compare the effects of melatonin, pentoxifylline, and dimethyl sulfoxide (DMSO), in hepatic ischemia-reperfusion injury with different methods such as biochemical/ultrastructural changes and hepatobiliary scintigraphy. Methods: Thirty rabbits were used in the Laboratory of Experimental Animals of Trakya University under appropriate conditions. Sham laparotomy and only ischemia reperfusion group were planned. They were used melatonin, pentoxifylline, and DMSO after ischemia-reperfusion in the other three groups. 6 rabbits were randomly selected for each group. Rabbits in all groups were subjected to liver scintigraphy. Following scintigraphy, 2 cm2 of liver tissue was removed to examining for liver antioxidant enzyme levels (superoxide dismutase [SOD] and glutathione peroxidase [GPx]) and for liver electron microscopy. Results: Pentoxifylline and melatonin protected significantly uptake and excretion functions in liver scintigraphy. When the effects of all three substances were examined by electron microscopy, it was found that the three substances protected the liver from the effects of ischemia-reperfusion damage at varying rates. All three agents were found to protect SOD and GPx from falling in various amounts. Conclusions: Studies to prevent ischemia-reperfusion injury, which may develop as a result of the Pringle maneuver applied to liver transplantations as well as to liver resections or liver injuries, still maintain their popularity. In our study, the effects of agents were identified in three different ways. Ischemia-reperfusion injury-reducing effect of pentoxifylline gave parallel results with three methods.
Authors and Affiliations
Zeliha Türkyılmaz, Ahmet Hatipoğlu, Mahmut Yüksel, Nurettin Aydoğdu, Gülara Hüseyinova
Keywords
Related Articles
- EP ID EP491811
- DOI 10.18621/eurj.421296
- Views 117
- Downloads 0
The relationship between psychopathology, psychosocial adjustment, social support and quality of life in multiple sclerosis
Objectives: Multiple Sclerosis (MS) is a chronic disease which affects physical,psychological and social functioning of patients. In this study we aim to indicate psychiatric comorbidity, some psychosocial factors and th...
Conglutinatio cervicis uteri orifici externi: a rare disorder of uterine cervix
Cervical conglutination is quite rare complication of labor that can be misinterpreted as fully dilated totally effaced intact amniotic membrane, and in inexperienced hands trying to open the membrane results in the cata...
Re-expansion pulmonary edema after pleurocan catheterization: a case report
Re-expansion pulmonary edema (RPE) is a rare but potentially hazardous complication following evacuation of the pleural region. Symptomatic RPE occurs in less than 1% of patients after pleural drainage. Early diagnosis a...
Determination of minimum serum concentration to develop scaffold free micro-tissue
Objective. Formation of three-dimensional (3D) micro-tissues without scaffolds are widely used not only to define in vivo tissue formation mechanisms but also the development of different tissue-specific drugs. However,...
Hysteroscopy before the first in vitro fertilization: a 7-year experience from a single center
Objective. This study aims to evaluate the importance of performing hysteroscopy prior to the first attempt of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) by specifying the incidence of intrau...