Comparison of effects of melatonin, pentoxifylline and dimethyl sulfoxide in experimental liver ischemia-reperfusion injury by three different methods
Journal Title: The European Research Journal - Year 2019, Vol 5, Issue 1
Abstract
Objectives: Liver transplantation is increasingly being used in the treatment of end-stage liver disease. Ischemia-reperfusion injury is one of the major problems encountered in transplantation. In this study, we aimed to compare the effects of melatonin, pentoxifylline, and dimethyl sulfoxide (DMSO), in hepatic ischemia-reperfusion injury with different methods such as biochemical/ultrastructural changes and hepatobiliary scintigraphy. Methods: Thirty rabbits were used in the Laboratory of Experimental Animals of Trakya University under appropriate conditions. Sham laparotomy and only ischemia reperfusion group were planned. They were used melatonin, pentoxifylline, and DMSO after ischemia-reperfusion in the other three groups. 6 rabbits were randomly selected for each group. Rabbits in all groups were subjected to liver scintigraphy. Following scintigraphy, 2 cm2 of liver tissue was removed to examining for liver antioxidant enzyme levels (superoxide dismutase [SOD] and glutathione peroxidase [GPx]) and for liver electron microscopy. Results: Pentoxifylline and melatonin protected significantly uptake and excretion functions in liver scintigraphy. When the effects of all three substances were examined by electron microscopy, it was found that the three substances protected the liver from the effects of ischemia-reperfusion damage at varying rates. All three agents were found to protect SOD and GPx from falling in various amounts. Conclusions: Studies to prevent ischemia-reperfusion injury, which may develop as a result of the Pringle maneuver applied to liver transplantations as well as to liver resections or liver injuries, still maintain their popularity. In our study, the effects of agents were identified in three different ways. Ischemia-reperfusion injury-reducing effect of pentoxifylline gave parallel results with three methods.
Authors and Affiliations
Zeliha Türkyılmaz, Ahmet Hatipoğlu, Mahmut Yüksel, Nurettin Aydoğdu, Gülara Hüseyinova
Keywords
Related Articles
- EP ID EP491811
- DOI 10.18621/eurj.421296
- Views 107
- Downloads 0
Neovascular age-related macular degeneration: 18-month outcomes of aflibercept treatment in patients resistant to ranibizumab
Objectives: Aim of this study is to investigate the effect of intravitreal aflibercept therapy in an 18-month period in patients with recurrent neovascular age-related macular degeneration resistant to intravitreal ranib...
Examination of surgical and conservative treatment effects on depression of patients with moderate carpal tunnel syndrome
Objective: The aim of this study is to examine the outcomes of conservative and surgical treatments of patients with carpal tunnel syndrome as well as the effects of these two treatment options on depressive symptoms of...
The effect of levosimendan on Tp-e interval, Tp-e/QT ratio, Tp-e/QTc ratio and QRS interval in heart failure patients
Objectives. Increased Tp-e interval (from the peak-to-the-end of the electrocardiographic T wave) and Tp-e/QT ratio are associated with malignant ventricular arrhythmias. We aimed to evaluate the acute effects of levosim...
Determination of minimum serum concentration to develop scaffold free micro-tissue
Objective. Formation of three-dimensional (3D) micro-tissues without scaffolds are widely used not only to define in vivo tissue formation mechanisms but also the development of different tissue-specific drugs. However,...
MiR-33a and statins collaboratively reduce the proliferative capacity of prostate cancer cells
Objectives: Prostate cancer (PCa) is one of the leading causes of cancer deaths among men in the developed countries. Accumulating data suggests a high-cholesterol Western diet as an important risk factor for PCa. Beside...