Comparison of efficacy of various stains and immunohistochemistry in diagnosis of amoebic colitis
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 1
Abstract
Background: Amoebic colitis is a common parasitic infection especially in developing countries. It’s a devastating disease and should be diagnosed accurately. This study was designed to analyse compare efficacy & reliability of Haematoxylin & Eosin (H&E), Periodic acid schiff’s (PAS), Masson trichrome (MT)and immunohistochemical (IHC) marker in diagnosis of amoebic colitis, and to ascertain advantages and disadvantages of each stain used in amoebic colitis. Material & Methods: We studied 30 cases reported as amoebic colitis on H&E and 10 cases of non-specific colitis. The pick-up rate of trophozoites of Entamoeba histolytica in H&E, PAS, MT and IHC was compared. Observation & Results: Out of 30 cases reported as amoebic colitis on H&E, trophozoites were identified in 26 cases of fresh H&E stained slides, in 23 cases with PAS, in 15 cases with MT and 20 out of 30 cases were positive for trophozoites on IHC. Number of trophozoites identified was 5 times on IHC and 2 times on PAS in comparison to H&E. Whereas role of MT was found to be less significant in diagnosing amoebic colitis. Conclusion: IHC was found to be superior in offering accurate diagnosis of amoebic colitis as it is highly efficacious and gives intense brown colour which makes recognition of trophozoites easier. Our study results suggest that by using IHC marker for amoebic colitis, even complicated cases can be diagnosed easily where they are difficult to be identified using other stains. Another advantage of using IHC is that it curtails false positive diagnosis of amoebiasis on H&E
Authors and Affiliations
Dr Preeti Diwaker
Keywords
Related Articles
- EP ID EP501943
- DOI -
- Views 63
- Downloads 0
Comparison of Pre and Post Operative Corneal Astigmatism following Pterygium Excision and Conjunctival Autograft
Objective: To evaluate the effect of pterygium excision on pterygium induced corneal astigmatism Methods: Hospital based study conducted in a tertiary care centre in Western Maharashtra from August 2014 to March 2015. A...
Management of Chronic Plantar Fasciitis using Hyperosmolar Dextrose Injection
Plantar fasciitis is most common cause of heel pain. More than 90% of patients are managed by conservative treatment. Chronic recalcitrant cases of plantar fasciitis not responding to other treatment modalities can be ma...
Study of Vitamin D3 Profile in Essential Hypertension
Hypertension is the most common of the cardiovascular diseases, which is theleading cause of morbidity and mortality in the industrial world as well as becoming an increasing common disease in the developing countries. H...
Antibiograms of Orthopedic Wound Discharge Bacteria Isolated from a tertiary care teaching hospital
Diseases are inescapable in various orthopedic methods. Notwithstanding all aseptic precautionary measures, noteworthy postoperative bleakness, mortality, delayed healing center remain and expanded clinic costs are watch...
Rhinocerebral-Orbital Mucormycosis: Salvageable with Prompt Recognition and Treatment
A case of 10 yrs. old type 1 diabetic male, who presented with left eye orbital cellulitis and lid necrosis. After promptdiagnosis of Rhinocerebral orbital mucormycosis the patient was debulked and was started with i.v a...