Comparison of Filter Paper and Gelfoam as Templates for Orientation of Endoscopic Duodenal Biopsies
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 2
Abstract
ABSTRACT Introduction: Proper orientation of endoscopic mucosal biopsies is crucial for accurate histomorphologic assessment. Aim: To compare the morphology on two different templates, filter paper and gelfoam for orientation of biopsies. Materials and Methods: In this study a total of 21 duodenal biopsies were studied which were taken from the same site, fixed and oriented on wet filter paper and gelfoam in formalin, from patients who presented with malabsorptive symptoms. Histomorphological parameters studied were villous architecture, crypt architecture, intraepithelial lymphocyte (IEL) count per 100 enterocytes, villous tip IEL count per 20 enterocytes, inflammatory cells in lamina propria. Statistical analysis was done using IBM- SPSS software version 21. p-value <0.05 was considered as statistically significant. Results: Twenty-one biopsies were taken in both the templates. Authors had 18 biopsies for analysis as three sections on gelfoam were lost during processing. All the histomorphological parameters were studied and it was similar on both the templates. The level of agreement by kappa statistics was significant with kappa value of 0.727 for villous architecture, 0.852 for lamina propria inflammatory infiltrate, 1 for IEL and eosinophils in lamina propria with p-value of <0.001. Conclusion: Authors concluded, gelfoam or filter paper serves as a good template for proper orientation of tiny mucosal biopsies. However, further studies are needed on larger sample size to validate this finding.
Authors and Affiliations
Priyavadhana Balasubramanian, Bhawana Ashok Badhe, Rajesh Nachiappa Ganesh, Lakshmi C Panicker, Pazhanivel Mohan
Keywords
Related Articles
- EP ID EP558004
- DOI 10.7860/JCDR/2019/37550.12603
- Views 79
- Downloads 0
The Potential Role of Social Media in Pharmacovigilance in Nepal: Glimpse from a Resource-limited Setting
ABSTRACT Introduction: An ever-increasing trend in the use of social media offers a potential forum for exchanging information in developing countries. Easy access, quick dissemination and widespread use necessitate the...
Retrosternal Goitre-An Anaesthetic Challenge
ABSTRACT Airway management of patients undergoing thyroidectomy for Retrosternal Goitre (RSG) poses a unique challenge. Associated co-morbidities, manipulation of the airway by the surgeons, airway compromise during indu...
Effect of Ketofol on Pain and Complication after Caesarean Delivery under Spinal Anaesthesia: A Randomized Double-blind Clinical Trial
Introduction: Pain is the key concern of women after caesarean delivery that may interfere with breastfeeding. Aim: The aim of this study was to assess effect of ketofol (ketamine/propofol combination) on pain and compli...
Caries Detection with ICDAS and the WHO Criteria: A Comparitive Study
Introduction: Dental caries is the most prevalent chronic disease affecting mankind and is the primary reason for tooth pain and edentulism. The presence or absence of caries depends on the diagnostic cut-off points sele...
Immediate Outcome of Balloon Mitral Valvuloplasty with JOMIVA Balloon during Pregnancy
Introduction: Rheumatic mitral stenosis is the most common Valvular Heart Disease encountered during pregnancy. Balloon Mitral Valvuloplasty (BMV) is one of the treatment option available if the symptoms are refractory t...