Comparison of identification methods of vancomycin-resistant enterococci in MALDI-TOF MS, Vitek 2 and API 20 STREP
Journal Title: Postępy Nauk Medycznych - Year 2015, Vol 28, Issue 4
Abstract
Introduction. From the clinical point of view, differentiation of the following species in the genus Enterococcus is the most important: E. faecium and E. faecalis (responsible for over 90% of all enterococcal infections) and E. gallinarum, E. casseliflavus, E. flavescens due to their resistance to low concentrations of vancomycin (VanC phenotype). During research of enterococci strains resistant to vancomycin (VRE), we are observed some of those strains isolated from clinical specimens from hospitalized patients were identified by systems based on biochemical identification: Vitek® 2 and Api 20 STREP (bioMereiux, France) as E. gallinarum while other methods demonstrated that they were vancomycin resistant E. faecium (VRE).Aim. Aim of study was to compare system Vitek2 and Api 20 STREP vs MALDI-TOF MS for enterococci identification.Material and methods. 100 strains of enterococci were identified for species in systems based on biochemical reactions (system Vitek® 2 and API 20 STREP) and mass spectrometry (MALDI-TOF). The detection of ddl and vanC genes was the reference method.Results. The differences in identification of enterococci ranged from 2% in Vitek® 2 system to 6% for ATBTM Expression system in relation to MALDI – Biotyper.Conclusions. High cost and time-consuming of molecular methods, exclude them from the application in routine laboratory diagnosis. However consistency of results in species identification in mass spectrometry MALDI-TOF with genetic methods, abet to use its in routine microbiological diagnosis.
Authors and Affiliations
Łukasz Chabros, Robert T. Kuthan, Anna Sawicka-Grzelak, Grażyna Młynarczyk
Keywords
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