COMPARISON OF IMMUNOCHROMATOGRAPHIC ASSAY WITH BIOCHEMICAL TESTS FOR MYCOBACTERIAL ISOLATES
Journal Title: Journal of Evidence Based Medicine and Healthcare - Year 2015, Vol 2, Issue 35
Abstract
Tuberculosis is considered to be a major cause of death among the infectious diseases prevalent in India. Differentiation of M. tuberculosis from Nontuberculous Mycobacteria (NTM) is very important in the management of the disease. Recently, Standard Diagnostics (SD Yongin Korea) developed a simple and rapid assay using a mouse monoclonal anti-MPT64 antibody to discriminate between M. tuberculosis and NTM by Immunochromatography. These tests were found to have good sensitivity and specificity. METHOD: 200 sputum samples were processed and inoculated on conventional Lowenstein Jensen culture medium. The Mycobacterial isolates were subjected to biochemical tests (Niacin test and Nitrate reduction test) and Immunochromatographic assay. The isolates were also tested by Polymerase chain reaction. H37 RV strain was used as a positive control. RESULT: The culture isolates showed positive in 47 (23.5%) cases. 44 isolates were positive and 3 were negative by both biochemical tests and immunochromatographic assay. Similar results were obtained by PCR. The sensitivity and specificity of Immunochromatography test when compared to biochemical tests and PCR was 100%. Positive predictive value and Negative predictive values were 100%. CONCLUSION: Immunochromatographic assay showed comparable results with biochemical tests and this assay can be used as an alternative to biochemical tests for the confirmation of Mycobacterium tuberculosis complex, which avoids use of hazardous chemicals (Cyanogen bromide).
Authors and Affiliations
Ravish Kumar M. , Manjunath S. M. , Shruthi N, Nagesh Raju G.
Keywords
Related Articles
- EP ID EP231795
- DOI 10.18410/jebmh/2015/742
- Views 100
- Downloads 0
CYTOLOGICAL DIAGNOSIS OF PROLIFERATING PILOMATRICOMA MIMICKING MALIGNANCY
Proliferating pilomatricoma is a rare distinctive proliferative variant of pilomatricoma. It is a benign cystic neoplasm characterized histopathologically by matrical differentiation. Till date very few cases of prolifer...
A RETROSPECTIVE STUDY OF BLOOD GROUPS IN HEAD AND NECK MALIGNANCIES
BACKGROUND Malignancy is a unique disease with abnormal proliferation of cells with the properties of invasion, anaplasia and metastasis. It accounts for around 12% of the deaths throughout the world. Blood does vital ta...
STUDY OF CENTRAL VENOUS CATHETER RELATED BLOOD STREAM INFECTIONS IN PATIENTS ON HAEMODIALYSIS
BACKGROUND Temporary and permanent central venous catheters are used in majority of patients of CKD when initiated on hemodialysis and mostly these catheters act as bridge before permanent AV fistula assess could be obta...
VALSALVA LIKE RETINOPATHY SECONDARY TO LEUKEMIA: A CASE REPORT
Pre-retinal haemorrhages (Sub-ILM or Sub-hyaloid) are seen to be associated with valsalva retinopathy, Tersons syndrome, blood dyscrasias and blunt trauma. However spontaneous Sub-ILM/Sub-hyaloid haemorrhage is a rare pr...
STUDY OF EFFICACY TOPICAL TACROLIMUS IN DIFFERENT DERMATOSES
: Immunological diseases of the skin are serious concern to dermatologist all over the world. Treatment of such diseases is big challenge and topical immune modulators have been proved to be effective in this. Various an...