Comparison of in vitro Resistance of Wild Leishmania İsolates, Which are Resistant to Pentavalent Antimonial Compounds, Against Drugs Used in the Treatment of Leishmaniasis
Journal Title: Turkish Journal of Parasitology - Year 2020, Vol 44, Issue 1
Abstract
Objective: Meglumine antimoniate (Glucantime®) and Sodium stibogluconate (Pentostam®) are used for the treatment of cutaneous leismaniasis in Turkey. There is a reported resistance to these drugs in recent years. The aim of the present study was to compare the in vitro sensitivities of resistant Leishmania isolates against Amphotericin B, Miltefosine, Meglumine Antimoniate, Paromomycin and Sodium Stibogluconate. Methods: Five Leishmania isolates of patients with cutaneous leishmaniasis, who showed no clinical recovery despite two consecutive meglumine antimoniate treatments, which were stored in the Parasite Bank in Manisa Celal Bayar University Medical Faculty were selected. They were genotyped with Real-Time PCR using specific primers and probes to ITS1 region. Drug resistance levels of each Leishmania isolate were analysed for Amphotericin B, Miltefosine, Meglumine Antimoniate, Paromomycin, and Sodium Stibogluconate at concentrations of 500, 250, 125, 50, 25 μg/mL with XTT method and hemocytometer. Results: It was observed that the resistant Leishmania tropica isolates showed no resistance to Amphotericin B, and were sensitive to Miltefosine, Sodium Stibogluconate, Paromomycin and Meglumin Antimonate, respectively. In addition, Leishmania tropica (MHOM/AZ/1974/SAF-K27) isolate of the control group could stay viable in none of the drug concentrations of five agents in the study. Conclusion: It was determined that none of the selected resistant L. tropica isolates showed resistance to Amphotericin B and that was also shown statistically (p<0.05). The results of this study are important in guiding clinicians and researchers who conduct studies on drugs and search for new drug molecules.
Authors and Affiliations
Ahmet Özbilgin, İbrahim Çavuş, Tuğba Kaya, Ahmet Yıldırım, Mehmet Harman
Keywords
Related Articles
- EP ID EP685171
- DOI 10.4274/tpd.galenos.2019.6661
- Views 403
- Downloads 0
Cutaneous Leishmaniasis in Harrankapı, Şanlıurfa
No summary
First Report of a Troglostrongylus brevior Case in a Domestic Cat in Turkey
Parasitic bronchopneumonia plays an important role, especially in the respiratory diseases of cats. Troglostrongylus species have been reported as lung parasites of wild cats; however, in recent years, they have also bee...
Temperature and Time Dependent Variations in Anti-Toxoplasma gondii Antibody Titrations in Serum Samples
Objective: This study aimed to determine the temperature- and time-dependent variations in the anti-Toxoplasma gondii (T. gondii) antibody titres in serum samples collected from T. gondii-infected patients. Methods: Dur...
Demodex Infestation in Patients with Rosacea
Objective: Although the etiology of rosacea is not known exactly, it is thought as a multifactorial disease. Demodex spp. are asymptomatic saprophytic ectoparasites living in hair follicles and sebaceous glands. The aim...
Distribution of Intestinal Parasites Detected between September 2011-2018 at Dokuz Eylül University Medical Faculty Hospital
Objective: Intestinal parasitic diseases are important public health problems in our country as well as in the world. In this study, intestinal parasites were investigated in patients admitted to Dokuz Eylül University H...