Comparison of Indices of Insulin Resistance and Islet Beta-Cell Dysfunction across Rat Models of Diabetes Mellitus Induced by Modified Diets or Streptozotocin
Journal Title: Journal of Krishna Institute of Medical Sciences University - Year 2018, Vol 7, Issue 2
Abstract
Background: Induction of insulin resistance in rodents involves the use of Streptozotocin (STZ) or diets high in sucrose, fat or fructose; but the relative degrees of insulin resistance induced by each of these approaches are unclear. Aim and Objectives: We therefore compared the degree to which intraperitoneal STZ with or without high-fat or high-fructose diet would induce insulin resistance, glucose intolerance and islet β-cell dysfunction in Wistar rats. Materials and Methods: Subsets of STZ-injected rats administered streptozotocin at 30 mg/kg body weight for five successive days were fed normal diet (STZ), or diets high in fat or fructose for 30 or 60 days. Normoglycaemic rats on normal rodent chow, High Fat Diet (HFD) or High Fructose Drink (HFrD) constituted the Control (CTR), HFD or HFrD groups, respectively. Rats were anaesthetized and sacrificed at 30 or 60 days of high fat or fructose feeding followed by measurement of fasting plasma glucose and insulin; and calculation of the HOMA-IR and HOMA-%β. Oral Glucose Tolerance Test (OGTT) was done 48 hours prior to killing the animals. Results: Glucose tolerance and islet β-cell function were most severely perturbed in the STZ-injected hyperglycaemic rats fed diets high in fructose or fat, as indicated by the significantly increased (p<0.05) HOMA-IR or decreased HOMA- %β (p<0.05) at 30 or 60 days compared with the CTR, STZ or diet-only groups. Weekly blood glucose was most markedly and significantly (p<0.05) elevated in these same (STZ+diet) groups, with impaired OGTT. Conclusion: The profound impairment of glucose tolerance and β-cell function in the STZ-induced hyperglycaemic rats fed high-fat or high-fructose diet support the continued use of such models in the characterization of the molecular events associated with insulin resistance, and the testing of novel therapeutic interventions.
Authors and Affiliations
Oluwole Busayo Akinola, Sikiru Abayomi Biliaminu, Emmanuel Olusola Yawson, Michael Oluwatosin Gabriel
Keywords
Related Articles
- EP ID EP280458
- DOI -
- Views 71
- Downloads 0
Significance of Mast Cell Density and Distribution in Various HistopathologicalLesions of Leprosy
Background: Leprosy also known as Hansen’s disease(HD), is a chronic infection caused by the Mycobacteriumleprae. Mast cells in leprosy have been investigatedin the recent past and are being examinedas a basis for future...
Zika Virus: An Emerging Public Health Challenge
Emerging infectious diseases comprise a substantial proportion of global morbidity and mortality. The world has been hit by Zika virus (ZIKV) after it was able to surmount an effective public health response for its cont...
Moving Medical Education into the 21 Century: Commentary on A WorldwideChallenge
Prevalence of Dermatophytes in a Tertiary Care Center of Solapur, Maharashtra
Background: Skin infection due to dermatophytes has become a significant health problem in tropical and sub tropical countries including India. Aim & Objectives: The present study was undertaken with an aim to stu...
Relevance of Vascular Sciences Research
-