Comparison of Indices of Insulin Resistance and Islet Beta-Cell Dysfunction across Rat Models of Diabetes Mellitus Induced by Modified Diets or Streptozotocin
Journal Title: Journal of Krishna Institute of Medical Sciences University - Year 2018, Vol 7, Issue 2
Abstract
Background: Induction of insulin resistance in rodents involves the use of Streptozotocin (STZ) or diets high in sucrose, fat or fructose; but the relative degrees of insulin resistance induced by each of these approaches are unclear. Aim and Objectives: We therefore compared the degree to which intraperitoneal STZ with or without high-fat or high-fructose diet would induce insulin resistance, glucose intolerance and islet β-cell dysfunction in Wistar rats. Materials and Methods: Subsets of STZ-injected rats administered streptozotocin at 30 mg/kg body weight for five successive days were fed normal diet (STZ), or diets high in fat or fructose for 30 or 60 days. Normoglycaemic rats on normal rodent chow, High Fat Diet (HFD) or High Fructose Drink (HFrD) constituted the Control (CTR), HFD or HFrD groups, respectively. Rats were anaesthetized and sacrificed at 30 or 60 days of high fat or fructose feeding followed by measurement of fasting plasma glucose and insulin; and calculation of the HOMA-IR and HOMA-%β. Oral Glucose Tolerance Test (OGTT) was done 48 hours prior to killing the animals. Results: Glucose tolerance and islet β-cell function were most severely perturbed in the STZ-injected hyperglycaemic rats fed diets high in fructose or fat, as indicated by the significantly increased (p<0.05) HOMA-IR or decreased HOMA- %β (p<0.05) at 30 or 60 days compared with the CTR, STZ or diet-only groups. Weekly blood glucose was most markedly and significantly (p<0.05) elevated in these same (STZ+diet) groups, with impaired OGTT. Conclusion: The profound impairment of glucose tolerance and β-cell function in the STZ-induced hyperglycaemic rats fed high-fat or high-fructose diet support the continued use of such models in the characterization of the molecular events associated with insulin resistance, and the testing of novel therapeutic interventions.
Authors and Affiliations
Oluwole Busayo Akinola, Sikiru Abayomi Biliaminu, Emmanuel Olusola Yawson, Michael Oluwatosin Gabriel
Keywords
Related Articles
- EP ID EP280458
- DOI -
- Views 55
- Downloads 0
Anaesthesia in Congenital Facial Anomalies in a Rural Set up of a Developing Country
Background: India has an estimated backlog of 1000000 cleft patients. A total of 35000 new cleft patients are born each year. With the capacity to operate on approximately 50000 patients each year only 15000 patients...
Study of Candida Bloodstream Infections in Surgical Intensive Care Unit Patients and Susceptibility Profile of the Isolates
Background: The increased incidence of fungal infections in the past two decades has been overwhelming. Despite the fact that invasive fungal infections are still under-diagnosed and underreported, bloodstream infect...
Revolutionizing Traditional Pathology Practice in Today’s Computer Age
Technology for acquisition of virtual slides was developed in 1985; however, it was not until the late 1990s that desktop computers had enough processing speed to commercialize virtual microscopy and apply the technology...
Adolescent Sexual and Reproductive Health: From Fallen Heroes to Future Leaders
Case Control Study to Assess Association between Periodontal Infection and Coronary Heart Disease
Background: Coronary heart disease is the leading cause of adult mortality and morbidity throughout the world. Well known risk factors independently or combined are involved in both atherosclerosis and myocardial infarct...