Comparison of L-Dopa content in three species of genus Mucuna by differente techniques
Journal Title: Annals of Plant Sciences - Year 2018, Vol 7, Issue 1
Abstract
In the present study, attempts are made to develop suitable method(s) for extraction of L-DOPA from the powdered seeds of 3 species of Mucuna using different solvents and conditions. The seed powder of both plants was subjected to 6 different extraction methods, with different solvent ratios. All the extracts were analyzed using RP-HPLC and were validated according to The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use guidelines. The L-DOPA extraction was best with Methanol Water mixture in a cold maceration technique and overall gives good extraction efficiency in all the three plants giving concentrations of 5.03%, 13.36 % and 16.78% of L-DOPA in Mucuna gigantea, Mucuna pruriens and Mucuna monosperma, respectively. The present investigation was done to study the extraction efficiency of various extraction methods of L-DOPA content in seed extracts of Mucuna and compare it.
Authors and Affiliations
Ragni Vora, Ambika N. Joshi, Nitesh Chandulal Joshi
Keywords
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- EP ID EP280281
- DOI 10.21746/aps.2018.7.1.10
- Views 117
- Downloads 0
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