Comparison of Methods to Assay Liver Glycogen Fractions: The Effects of Starvation
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 3
Abstract
Introduction: There are several methods to extract and measure glycogen in animal tissues. Glycogen is extracted with or without homogenization by using cold Perchloric Acid (PCA). Aim: Three procedures were compared to determine glycogen fractions in rat liver at different physiological states. Materials and Methods: The present study was conducted on two groups of rats, one group of five rats were fed standard rodent laboratory food and were marked as controls, and another five rats were starved overnight (15 hour) as cases. The glycogen fractions were extracted and measured by using three methods: classical homogenization, total-glycogenfractionation and homogenization-free protocols. Results: The data of homogenization methods showed that following 15 hour starvation, total glycogen decreased (36.4±1.9 vs. 27.7±2.5, p=0.01) and the change occurred entirely in Acid Soluble Glycogen (ASG) (32.0±1.1 vs. 22.7±2.5, p=0.01), while Acid Insoluble Glycogen (AIG) did not change significantly (4.9±0.9 vs. 4.6±0.3, p=0.7). Similar results were achieved by using the method of total-glycogen-fractionation. Homogenization-free procedure indicated that ASG and AIG fractions compromise about 2/3 and 1/3 of total glycogen and the changes occurred in both ASG (24.4±2.6 vs. 16.7±0.4, p<0.05) and AIG fraction (8.7±0.8 vs. 7.1±0.3, p=0.05). Conclusion: The findings of ‘homogenization assay method’ indicate that ASG is the major portion of liver glycogen and is more metabolically active form. The same results were obtained by using ‘total-glycogen-fractionation method’. ‘Homogenizationfree method’ gave different results, because AIG has been contaminated with ASG fraction. In both ‘homogenization’ and ‘homogenization-free’ methods ASG must be extracted at least twice to prevent contamination of AIG with ASG.
Authors and Affiliations
Nastaran Mojibi, mehdi rasouli
Keywords
Related Articles
- EP ID EP333991
- DOI 10.7860/JCDR/2017/24783.9555
- Views 116
- Downloads 0
Aortic Knob Width as an Indicator of Cardiovascular Risk in Chronic Kidney Disease Patients
ABSTRACT Introduction: The most common cause of mortality in Chronic Kidney Disease (CKD) patients is due to cardiovascular events. The Aortic Knob Width (AKW) has been found to positively correlate with the severity of...
Impact of Educational Intervention on Quality of Discharge Summaries
ABSTRACT Introduction: Immaculate and timely communication among healthcare providers is crucial for continuity of care. Discharge summaries are commonly used for communication among different healthcare facilities. Def...
Paracervical Block and Paracetamol for Pain Reduction During IUD Insertion: A Randomized Controlled Study
Introduction: Unintended pregnancies and its consequences are a considerable public health problem. Intrauterine Devices (IUDs) are reversible, safe and effective contraceptive method for most women. Aim: The aim of this...
Inflammation and Outcome in Traumatic Brain Injury: Does Gender Effect on Survival and Prognosis?
Introduction: Traumatic Brain Injury (TBI) accounts for the majority of trauma deaths and there has been increased interest in the understanding the role of prognostic factors. C-Reactive Protein (CRP) level increases ra...
Diagnosis of Lafora Disease by Skin Biopsy
Images in Medicine