Comparison of modified Kreyberg's, modified Papanicolaou, Ayoub-Shklar, and Haematoxylin and Eosin stains to demonstrate keratin in paraffin embedded tissue sections
Journal Title: Journal of Dental Specialities - Year 2017, Vol 5, Issue 2
Abstract
Introduction: Keratin is an intermediate protein with a diameter of 7-11 nm which forms the cytoskeleton of all the epithelial cells. It forms the superficial most layer (stratum corneum) in keratinized epithelium in the oral cavity. Keratin may also be present in various pathologic conditions like squamous cell carcinoma, verrucous carcinoma and odonogenic keratocyst. The aim of the present study was to compare and evaluate the efficacy of staining of Ayoub-Shklar, Modified Papanicolaou, Routine Haematoxylin and Eosin and Modified Kreyberg's to stain known keratin tissue, and to obtain the best stain for keratin. Materials and Method: A total of 75 paraffin embedded tissue blocks were taken of known keratin containing tissue which included Well Differentiated Squamous Cell Carcinoma (WDSCC), Hyperkeratosis with or without dysplasia (HWD), Orthokeratinized Odontogenic Keratocyst (OKC) and Verrucous Carcinoma (VC). Four sections measuring 4 microns in thickness of each block were cut and were stained with all the mentioned stains. Results: Keratin was stained by all the four stains (Ayoub-Shklar, Modified Papanicolaou, Routine Haematoxylin and Eosin and Modified Kreyberg's) distinctly in WDSCC, HWD, OKC and VC. Ayoub-Schklar and Hematoxylin and Eosin stains showed better results, and was also statistically significant among all the stains for WDSCC, HWD, OKC and VC when intensity and patterns were compared. Conclusion: It can be concluded that all the four stains (Ayoub-Shklar, Modified Papanicolaou, Routine Haematoxylin and Eosin and Modified Kreyberg's) can demonstrate keratin and based on the overall staining intensity and pattern. Ayoub-Shklar and H and E stains can be used efficiently to stain keratin but have certain limitations.
Authors and Affiliations
Aprajita Srivastava, Anil Singh, Kunal Sah, Vineet Raj, Bhavana Gupta
Keywords
Related Articles
- EP ID EP299164
- DOI 10.18231/2393-9834.2017.0031
- Views 53
- Downloads 0
Effect of educational status on oral health education program amongst Anganwadi workers in improving oral health of preschool children of Muradnagar block, Ghaziabad-A cross-sectional study
Aim To study effect of educational status of Anganwadi Workers on oral health education program and its impact on improving oral health of preschool children in Muradnagar block GhaziabadMaterials and Methods Study was d...
Use of a semi-precision attachment to fabricate a removable partial denture – a case report
Use of attachments in providing better retention to removable prosthesis is not an outdated treatment modality. It is important for dentists to have more updated knowledge about its use and the success of such prosthesis...
Follicular-acanthomatous ameloblastoma and impact on intervention
Ameloblastoma is the second most common benign neoplasm of odontogenic origin It occurs commonly in third to fifth decade of life with no sex predilection The clinical presentation of tumor includes swelling and facial d...
Evaluation of frictional forces generated in retrieved aesthetic wires with different ligation methods
Objective: To evaluate frictional forces generated in retrieved aesthetic archwires with different ligation methods. Materials and Method: A total of 40(20 new and 20 retrieved from the patient’s mouth) Teflon coated 019...
Veneers in esthetic zone – A case series
With the growth of cosmetic dental procedures the practise of dentistry has become increasingly centred on fulfilling patient desires Porcelain veneers are increasing in popularity among todays patients and dental practi...