Comparison of Molecular Methods of Microbial Serotyping
Journal Title: Microbiology Research Journal International - Year 2015, Vol 7, Issue 1
Abstract
Molecular serotyping methods have advantages and disadvantages in terms of different parameters. Since different methods depend on different parameters there is a possibility that one serotype detected by one method maybe missed by another method. So it is very important to use combination of molecular methods for serotyping because it would allow accurate serotyping. The best molecular serotyping method is pyrosequencing. However, advanced molecular methods, especially the sequence-based methods are currently evolving and relatively expensive; it would take some time for it to be widely used. To increase the serotyping capacity of Polymerase Chain Reaction (PCR), more serotype-specific primers should be designed. To increase the reliability of DNA microarray, an internal probe hybridization control (IHC) can be used which would indicate any variability in the hybridization process. Moreover to further decrease the cost of DNA microarray, all fluorescent labelling can be replaced with biotin labelling. Sequencing of more isolates of the same serotype would definitely improve the sequence-based serotyping assays.
Authors and Affiliations
Archana Singh Sikarwar, Mariyam Nashtha Nashid
Keywords
Related Articles
- EP ID EP354759
- DOI 10.9734/BMRJ/2015/15210
- Views 100
- Downloads 0
Co-infections; Their Role in HIV Acquisition and Disease Progression
HIV/AIDS disease manifestations play critical roles on the host's immune response to infections especially cell-mediated immunity which is central in combating many other infections, allowing opportunistic pathogens that...
Endophytic Microorganisms in Leaves of Moringa oleifera Collected in Three Localities at Pernambuco State, Northeastern Brazil
Aims: This work investigated the population density of endophytic microorganisms from Moringa oleifera leaves collected in three localities at the state of Pernambuco (northeastern Brazil): Urban (campus from the...
Virulence of Fusarium oxysporum on Kidney Organ Using In vivo Mice Model
Aim: To determine the virulence of Fusarium oxysporum on mice kidney organ. Study Design: Study was carried out using experimental design. Place and Duration: The study was carried out at University of Nigeria, Enugu Ca...
Evaluation of Bio-insecticidal Property of Bacillus thuringiensis Strains Isolated from Western Ghats Soil
Morphological and cultural characterization of the native twenty isolates obtained from Western Ghats of Kerala, produced creamy white and puffy colonies. The colonies were circular or irregular and flat. Margins were ei...
Antibacterial Resistance Profile and PCR Detection of Antibiotic Resistance Genes in Salmonella serovars Isolated from Blood Samples of Hospitalized Subjects in Kano, North-West, Nigeria
Aim: The aims of the study were to determine the antibacterial resistance profile and detect the presence of antibacterial resistance genes of Salmonella isolates recovered from the blood samples of hospitalized subjects...