Comparison of Real time PCR with traditional tools for diagnosis of Trichomonas vaginalis in patient with vaginal discharge at Kut city, Iraq
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 5, Issue 3
Abstract
Trichomoniasis is a sexually transmitted disease (STD) and most common form of STD which is caused by Trichomonas vaginalis. This is a descriptive analytical study. Vaginal swabs were collected from sixty female patients with suspected Trichomonas vaginalis infection aged 14-53 years old during September 2013 to March 2014 who attended the Gynecology Clinic of Kut city, Iraq. Two swabs were collected from each woman; one for wet mount microscopic examination and the other for Real Time PCR technique. Wet-mount microscopy were positive for T. vaginalis in 5/60 cases (8.33%) and positive Real-time PCR 13/60 (21.67%). The present study revealed that the highest incidence of T.vaginalis infection occurs in age group(24-33) years with the percentage of (46.1%). The sensitivity of Real-time PCR was 100% compared with 38.5% for wet-mount microscopy. The aim of the study was to compare a Real-time PCR assay with wet-mount examination for the detection of T. vaginalis.
Authors and Affiliations
Abdulsadah Abdulabbas Rahi, Magda A Ali, Ali Khamesipour
Keywords
Related Articles
- EP ID EP654438
- DOI 10.24297/jab.v5i3.5563
- Views 208
- Downloads 0
Effect of Ground Seeds of Picralima nitida Incorporated Diets on Serum Lipid Profile of Albino Rats
The effect of ground seeds of Picralima nitida incorporated diets on serum lipid profile of albino rats was studied. Twenty five albino rats randomly distributed into five groups of five rats were fed with 5%, 10%, 20% a...
INFLUENCE OF THE AGE AND COMORBIDITY ON ORAL HEALTH AND QUALITY OF LIFE AT GERIATRIC POPULATION WITH TOTAL AND PARTIAL DENTURES IN REPUBLIC OF MACEDONIA
Health and health condition are basic components of quality of life, and oral health connective part of the general health. Age and comorbidity conditions are important co-factors for oral health and quality of life. Mai...
QUANTIFIED CHARACTERIZATION OF SOIL BIOLOGICAL ACTIVITY UNDER CROP CULTIVATION
Soil biological activity is extremely important for sustaining high soil fertility, crop yields, and therefore can effect economy. Poland is particularly relevant example where approx. 60% of land is used for crop cultiv...
P53 TRANSCRIPTION FACTOR AND DIABETES: IS THERE ANY LINK?
Diabetes, a metabolic disorder is alarmingly increasing across the globe and currently ample of research is being carried out to treat hyperglycaemia and its secondary complications in diabetic patients. Despite extensiv...
Testosterone Level, Testicular Histology and Reproductive Events of Caucasian Pit Viper, Gloydius Halys Caucasicus (Serpentes: Viperidae)
The steroid hormones regulate the reproductive physiology of animals and may influence by habitat and seasonal conditions. In this study, body weight and length, testosterone concentration and testicular histology were m...