Comparison of the analytical techniques of hba1c estimation by immunoturbidimetric and HPLC methods in diabetic and pre- diabetics patients
Journal Title: International Journal of Clinical Biochemistry and Research - Year 2017, Vol 4, Issue 2
Abstract
Aims & Objectives: The aim of the study is to compare the available analytical methods of measurement of HbA1c like immunoturbidimetric and HPLC methods. Materials and Method: Total of 150 pre-diabetic and diabetic patients are included in the study. HbA1c was measured by immunoturbidimetric and HPLC methods as these are the common methods used by the clinical laboratories. Results: The HbA1c values of two methods have a significant difference. The value of immunoturbidimetric method was less than the HPLC method. The two methods correlated with the correlation coefficient of 0.972. Conclusion: Both the methods are reliable and show a strong correlation .Even though the immunoturtbidimetric values are low it is specific and more cost effective than the HPLC methods. HPLC is a sensitive method.
Authors and Affiliations
A . G. Thivyah Prabha
Keywords
Related Articles
- EP ID EP296934
- DOI 10.18231/2394-6377.2017.0043
- Views 71
- Downloads 0
Evaluation of serum pancreatic enzymes in type 2 diabetes mellitus and its correlation with insulin resistance
Introduction: Type 2 diabetes mellitus is due to the decreased biological response to insulin or insulin resistance. Insulin resistance develops as a consequence of defects in signal transduction by insulin. These defect...
Perinatal complications in intrahepatic cholestasis of pregnancy
Background and Objective: Intrahepatic cholestasis of pregnancy (ICP) is seen usually in 3 rd trimester of pregnancy. Overall incidence is 1.2-1.5% of Indian pregnant women. The biochemical abnormalities often include ra...
Normative data of the urinary bone resorption biomarkers for osteoporosis DPD, CTx and NTx among females in Manipur and its correlation with bone mineral density measured by DEXA scan
Introduction Osteoporosis has become a major public health problem affecting more than 100 million people worldwide Standard diagnosis depends on measuring bone mineral density BMD using a dual energy Xray absorptiometry...
Relation between serum electrolytes & serum creatinine levels in diabetes mellitus
Aim: Diabetes mellitus with altered renal function is associated with the disturbed pattern in serum electrolytes. There arises a need to evaluate the serum electrolytes in diabetic patients according to the stage of the...
Dealing with seasonal pseudohypokalemia in paediatric patients due to high ambient temperature in tropical climate: A laboratory perspective
Introduction During summer 2015 we had observed a significant rise in incidence of hypokalemia in comparison to winter 20142015 We speculated these findings might have occurred as a result of delay in centrifugation duri...