Computational identification of miRNAs and their targets from Niger (Guizotia abyssinica)
Journal Title: Journal of Applied Biology & Biotechnology - Year 2017, Vol 5, Issue 2
Abstract
MicroRNAs play a pivotal role in regulating a broad range of biological processes, acting by cleaving mRNAs or by translational repression. A group of plant microRNAs are evolutionarily conserved; however, others are expressed in a species-specific manner. In this study we used homology-based analysis with available expressed sequence tag (EST) of Niger (Guizotia abyssinica) to predict conserved miRNAs. Two potent miRNAs targeting 49 genes were identified. The newly identified miRNAs belongs to miR2592 and miR396 family. Targets recognized were F-box proteins, leucine zipper, DEAD box RNA helicase, disease resistant proteins. Gene annotations revealed miRNAs were involved in growth and development and Encyclopaedia of Genes and Genomes (KEGG) pathway analyses showed miRNAs were involved in metabolic pathways.
Effect of polyamines on parental and hybrid strains of Bombyx mori
The silk produced by Bombyx mori, mulberry silk, is of very high economic importance. Many hybrid strains of Bombyx mori were developed to improve silk production and to withstand different environmental conditions. Poly...
Growth responses on major replacement of animal protein with plant protein and graded levels of dietary supplement amino sugar, glucosamine in threatened Magur (Clarias batrachus, Linnaeus, 1758) fry
A 12-week feeding trial was performed to evaluate the use of animal and plant protein, in combination with dietary supplement glucosamine (GlcN) an amino sugar and a prominent precursor in the biochemical synthesis of gl...
Cloning, expression and purification of resistance gene analogue RGPM 301 from pearl millet in Escherichia coli
Plants combat their pathogens with an array of defense responses. One of the key mechanisms involves products of resistance (R) genes which are responsible for recognition of effector molecules from pathogens and subsequ...
Utilization of molecular methods to relate Red Mark Syndrome affecting Oncorhynchus mykiss to an unculturable Rickettsiales organism
Red Mark Syndrome (RMS) is an inflammatory skin condition related to the presence of an unculturable Rickettsiales organism that affects farmed rainbow trout, Oncorhynchus mykiss. This disease is responsible of economic...
Isolation and characterization of denitrifying halophilic bacteria from Bahr Al-Milh Salt Lake, Karbala, Iraq
Nitrogen fixation is considered as a significant biological process, which is important in agricultural and environmental implications. Herein denitrifying bacteria from Bahr Al-Milh Salt Lake, Karbala, Iraq, was reporte...